Homo-fermentative production of d-lactic acid by Lactobacillus sp. employing casein whey permeate as a raw feed-stock
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Casein whey permeate (CWP), a lactose-enriched dairy waste effluent, is a viable feed stock for the production of value-added products. Two lactic acid bacteria were cultivated in a synthetic casein whey permeate medium with or without pH control. Lactobacillus lactis ATCC 4797 produced d-lactic acid (DLA) at 12.5 g l−1 in a bioreactor. The values of Leudking–Piret model parameters suggested that lactate was a growth-associated product. Batch fermentation was also performed employing CWP (35 g lactose l−1) with casein hydrolysate as a nitrogen supplement in a bioreactor. After 40 h, L. lactis produced 24.3 g lactic acid l−1 with an optical purity >98 %. Thus CWP may be regarded as a potential feed-stock for DLA production.
KeywordsCasein whey permeate d-Lactic acid Homo-fermentative Lactobacillus lactis Optical purity
List of symbols
Specific DLA productivity (g DLA g−1 dry cell weight l−1)
Growth-associated product constant
Non-growth-associated product constant
Specific growth rate (h−1)
Biomass yield coefficient (g DCW g−1 lactose)
Product yield coefficient (g DLA g−1 lactose)
Authors thank Dr. Chandan Das (Chemical Engineering Department, IIT Guwahati) for rendering valuable support to perform ultra-filtration of whey at his lab facility. Authors express their special thanks to Dr. Vimal Katiyar (Chemical Engineering Department, IIT Guwahati) for his valuable suggestions. The Authors thank Nitin More for critical evaluation of the text and grammatical corrections in the manuscript. This work was supported by Biotechnology Department, IIT Guwahati funded by Ministry of Human Resources and Development (MHRD), New Delhi, India.
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