Biotechnology Letters

, Volume 36, Issue 6, pp 1303–1307 | Cite as

Homo-fermentative production of d-lactic acid by Lactobacillus sp. employing casein whey permeate as a raw feed-stock

  • Saurav Prasad
  • Katla Srikanth
  • Anil M. Limaye
  • Senthilkumar SivaprakasamEmail author
Original Research Paper


Casein whey permeate (CWP), a lactose-enriched dairy waste effluent, is a viable feed stock for the production of value-added products. Two lactic acid bacteria were cultivated in a synthetic casein whey permeate medium with or without pH control. Lactobacillus lactis ATCC 4797 produced d-lactic acid (DLA) at 12.5 g l−1 in a bioreactor. The values of Leudking–Piret model parameters suggested that lactate was a growth-associated product. Batch fermentation was also performed employing CWP (35 g lactose l−1) with casein hydrolysate as a nitrogen supplement in a bioreactor. After 40 h, L. lactis produced 24.3 g lactic acid l−1 with an optical purity >98 %. Thus CWP may be regarded as a potential feed-stock for DLA production.


Casein whey permeate d-Lactic acid Homo-fermentative Lactobacillus lactis Optical purity 

List of symbols


Specific DLA productivity (g DLA g−1 dry cell weight l−1)


Growth-associated product constant


Non-growth-associated product constant


Specific growth rate (h−1)


Biomass yield coefficient (g DCW g−1 lactose)


Product yield coefficient (g DLA g−1 lactose)



Authors thank Dr. Chandan Das (Chemical Engineering Department, IIT Guwahati) for rendering valuable support to perform ultra-filtration of whey at his lab facility. Authors express their special thanks to Dr. Vimal Katiyar (Chemical Engineering Department, IIT Guwahati) for his valuable suggestions. The Authors thank Nitin More for critical evaluation of the text and grammatical corrections in the manuscript. This work was supported by Biotechnology Department, IIT Guwahati funded by Ministry of Human Resources and Development (MHRD), New Delhi, India.

Supplementary material

10529_2014_1482_MOESM1_ESM.docx (23 kb)
Supplementary material 1 (DOCX 22 kb)


  1. Ahn WS, Park SJ, Lee SY (2000) Production of poly (3-hydroxybutyrate) by fed-batch culture of recombinant Escherichia coli with a highly concentrated whey solution. Appl Environ Microbiol 66:3624–3627PubMedCentralPubMedCrossRefGoogle Scholar
  2. Barile D, Tao N, Lebrilla CB, Coisson JD, Arlorio M, German JB (2009) Permeate from cheese whey ultrafiltration is a source of milk oligosaccharides. Int Dairy J 19:524–530PubMedCentralPubMedCrossRefGoogle Scholar
  3. Fitzpatrick JJ, O’Keeffe U (2001) Influence of whey protein hydrolysate addition to whey permeate batch fermentations for producing lactic acid. Proc Biochem 37:183–186CrossRefGoogle Scholar
  4. Ghasemi M, Najafpour G, Rahimnejad M, Beigi PA, Sedighi M, Hashemiyeh B (2009) Effect of different media on production of lactic acid from whey by Lactobacillus bulgaricus. Afr J Biotech 8(1):081–084Google Scholar
  5. Martin K, Atlić A, Dias M, Reitererand A, Braunegg G (2010) Microbial PHA production from waste raw materials, plastics from bacteria: natural functions and applications. Microbiol Monogr 14:85–119CrossRefGoogle Scholar
  6. Otto RT, Daniel HJ, Pekin G, Müller-Decker K, Fürstenberger G, Reuss M, Syldatk C (1999) Production of sophorolipids from whey. Appl Microbiol Biotechnol 52:495–501PubMedCrossRefGoogle Scholar
  7. Ozmihci S, Kargi F (2007) Kinetics of batch ethanol fermentation of cheese-whey powder solution as function of substrate and yeast concentration. Biores Technol 98:2978–2984CrossRefGoogle Scholar
  8. Södergård A, Stolt M (2002) Properties of lactic acid based polymers and their correlation with composition. Prog Polym Sci 27(6):1123–1163CrossRefGoogle Scholar
  9. Tango MSA, Ghaly AE (1999) Amelioration of lactic acid production from cheese whey using micro-aeration. Biomass Bioenergy 17(3):221–238CrossRefGoogle Scholar
  10. Tashiro Y, Kaneko W, Sun Y, Shibata K, Inokuma K, Zendo T, Sonomoto K (2011) Continuous d-lactic acid production by a novel thermotolerant Lactobacillus delbrueckii subsp. lactis QU 41. Appl Microbiol Biotechnol 89(6):1741–1750PubMedCrossRefGoogle Scholar
  11. USDA United States Department of Agriculture (2004) Washington, D.C.: U.S. Dept. of Agriculture.
  12. Yoshito I, Jamshidi K, Tsuji H, Hyon SH (1987) Stereocomplex formation between enantiomeric poly(lactides). Macromolecule 20(4):904–906CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Saurav Prasad
    • 1
  • Katla Srikanth
    • 1
  • Anil M. Limaye
    • 1
  • Senthilkumar Sivaprakasam
    • 1
    Email author
  1. 1.Department of BiotechnologyIndian Institute of Technology, GuwahatiGuwahatiIndia

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