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Biotechnology Letters

, Volume 36, Issue 6, pp 1303–1307 | Cite as

Homo-fermentative production of d-lactic acid by Lactobacillus sp. employing casein whey permeate as a raw feed-stock

  • Saurav Prasad
  • Katla Srikanth
  • Anil M. Limaye
  • Senthilkumar SivaprakasamEmail author
Original Research Paper

Abstract

Casein whey permeate (CWP), a lactose-enriched dairy waste effluent, is a viable feed stock for the production of value-added products. Two lactic acid bacteria were cultivated in a synthetic casein whey permeate medium with or without pH control. Lactobacillus lactis ATCC 4797 produced d-lactic acid (DLA) at 12.5 g l−1 in a bioreactor. The values of Leudking–Piret model parameters suggested that lactate was a growth-associated product. Batch fermentation was also performed employing CWP (35 g lactose l−1) with casein hydrolysate as a nitrogen supplement in a bioreactor. After 40 h, L. lactis produced 24.3 g lactic acid l−1 with an optical purity >98 %. Thus CWP may be regarded as a potential feed-stock for DLA production.

Keywords

Casein whey permeate d-Lactic acid Homo-fermentative Lactobacillus lactis Optical purity 

List of symbols

qDLA

Specific DLA productivity (g DLA g−1 dry cell weight l−1)

α

Growth-associated product constant

β

Non-growth-associated product constant

μ

Specific growth rate (h−1)

YX/S

Biomass yield coefficient (g DCW g−1 lactose)

YP/S

Product yield coefficient (g DLA g−1 lactose)

Notes

Acknowledgments

Authors thank Dr. Chandan Das (Chemical Engineering Department, IIT Guwahati) for rendering valuable support to perform ultra-filtration of whey at his lab facility. Authors express their special thanks to Dr. Vimal Katiyar (Chemical Engineering Department, IIT Guwahati) for his valuable suggestions. The Authors thank Nitin More for critical evaluation of the text and grammatical corrections in the manuscript. This work was supported by Biotechnology Department, IIT Guwahati funded by Ministry of Human Resources and Development (MHRD), New Delhi, India.

Supplementary material

10529_2014_1482_MOESM1_ESM.docx (23 kb)
Supplementary material 1 (DOCX 22 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Saurav Prasad
    • 1
  • Katla Srikanth
    • 1
  • Anil M. Limaye
    • 1
  • Senthilkumar Sivaprakasam
    • 1
    Email author
  1. 1.Department of BiotechnologyIndian Institute of Technology, GuwahatiGuwahatiIndia

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