Abstract
Two encapsulation techniques for rabbit chondrocytes in chitosan/hyaluronic acid gel have been compared. The standard technique involves the cross-linking of chitosan and hyaluronic acid at 2:1 (w/w). In the modified technique, cells were initially added to 33 % of hyaluronic acid dialdehyde and the gelation process was completed with the remaining 67 %. This minimised the cell loss and improved the encapsulation of the cells. By the third week, the modified technique showed better seeding density, with matrix synthesis (per scaffold) of 11 μg as compared to 1.1 μg in the current technique. Relative expression of collagen II with the current technique and the modified technique were 6.4 % and ~1,600 % respectively. The modified technique was superior for matrix synthesis and maintenance of phenotype.
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References
Correia CR, Moreira-Teixeira LS, Moroni L, Reis RL, Van Blitterswijk CA, Karperien M, Mano JF (2011) Chitosan scaffolds containing hyaluronic acid for cartilage tissue engineering. Tissue Eng Part C Methods 17:717–730
Hwang NS, Varghese S, Elisseeff J (2007) Cartilage tissue engineering: directed differentiation of embryonic stem cells in three-dimensional hydrogel culture. Methods Mol Biol 407:351–373
McCullen SD, Chow AG, Stevens MM (2011) In vivo tissue engineering of musculoskeletal tissues. Curr Opin Biotechnol 22:715–720
Meretoja VV, Dahlin RL, Wright S, Kasper FK, Mikos AG (2013) The effect of hypoxia on the chondrogenic differentiation of co-cultured articular chondrocytes and mesenchymal stem cells in scaffolds. Biomaterials 34:4266–4273
Park H, Choi B, Hu J, Lee M (2012) Injectable chitosan/hyaluronic acid hydrogels for cartilage tissue engineering. Acta Biomater 9:4779–4786
Ponticiello MS, Schinagl RM, Kadiyala S, Barry FP (2000) Gelatin-based resorbable sponge as a carrier matrix for human mesenchymal stem cells in cartilage regeneration therapy. J Biomed Mater Res 52:246–255
Rajagopal K, Dutt V, Manickam AS, Madhuri V (2012) Chondrocyte source for cartilage regeneration in an immature animal: is iliac apophysis a good alternative? Indian J Orthop 46:402–406
Remya N, Nair PD (2012) Engineering cartilage tissue interfaces using a natural glycosaminoglycan hydrogel matrix—An in vitro study. Mater Sci Eng, C 30:575–582
Tan H, Chu CR, Payne KA, Marra KG (2009) Injectable in situ forming biodegradable chitosan–hyaluronic acid based hydrogels for cartilage tissue engineering. Biomaterials 30:2499–2506
Thomas CM, Fuller CJ, Whittles CE, Sharif M (2007) Chondrocyte death by apoptosis is associated with cartilage matrix degradation. Osteoarth Cartil 15:27–34
Acknowledgments
This study is a part of Indo-Danish collaborative project funded by Department of Biotechnology, Government of India and Danish Council for Strategic Research, Denmark. The authors acknowledge the Department of Nuclear Medicine, Christian Medical College, Vellore, for providing the lyophilisation facility.
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Ramesh, S., Rajagopal, K., Vaikkath, D. et al. Enhanced encapsulation of chondrocytes within a chitosan/hyaluronic acid hydrogel: a new technique. Biotechnol Lett 36, 1107–1111 (2014). https://doi.org/10.1007/s10529-014-1461-1
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DOI: https://doi.org/10.1007/s10529-014-1461-1