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A novel perfused rotary bioreactor for cardiomyogenesis of embryonic stem cells

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Abstract

Developments in bioprocessing technology play an important role for overcoming challenges in cardiac tissue engineering. To this end, our laboratory has developed a novel rotary perfused bioreactor for supporting three-dimensional cardiac tissue engineering. The dynamic culture environments provided by our novel perfused rotary bioreactor and/or the high-aspect rotating vessel produced constructs with higher viability and significantly higher cell numbers (up to 4 × 105 cells/bead) than static tissue culture flasks. Furthermore, cells in the perfused rotary bioreactor showed earlier gene expressions of cardiac troponin-T, α- and β-myosin heavy chains with higher percentages of cardiac troponin-I-positive cells and better uniformity of sacromeric α-actinin expression. A dynamic and perfused environment, as provided by this bioreactor, provides a superior culture performance in cardiac differentiation for embryonic stem cells particularly for larger 3D constructs.

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Abbreviations

HARV:

High-aspect rotating vessel

ρ :

Density of fluid

υ :

Viscosity of fluid

D mi :

Diffusion coefficient of glucose

Y i :

Mass fraction of glucose

J i :

Diffusion flux of glucose

R i :

Net production rate of glucose

S i :

User defined source from neighboring grids of glucose

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Acknowledgments

The authors would like to acknowledge the financial support provided by Nanyang Technological University (NTU)-Imperial joint PhD program and Singapore National Research Foundation (NRF) under the NRF-Technion Research Thrust (NRF-Technion/2012-SG#04).

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Authors and Affiliations

  1. School of Chemical & Biomedical Engineering, Nanyang Technological University, Singapore, Singapore

    Ailing Teo & Mayasari Lim

  2. Department of Chemical Engineering, Imperial College London, South Kensington Campus, London, UK

    Ailing Teo & Athanasios Mantalaris

  3. School of Chemical Engineering & Technology, Dalian University of Technology, Dalian, China

    Kedong Song

  4. Division of Bioengineering, Nanyang Technological University, Block N1.3, 70 Nanyang Drive, Singapore, 637457, Singapore

    Mayasari Lim

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  1. Ailing Teo
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  2. Athanasios Mantalaris
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  3. Kedong Song
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  4. Mayasari Lim
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Corresponding author

Correspondence to Mayasari Lim.

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Teo, A., Mantalaris, A., Song, K. et al. A novel perfused rotary bioreactor for cardiomyogenesis of embryonic stem cells. Biotechnol Lett 36, 947–960 (2014). https://doi.org/10.1007/s10529-014-1456-y

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  • DOI: https://doi.org/10.1007/s10529-014-1456-y

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