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Diverse expression levels of two codon-optimized genes that encode human papilloma virus type 16 major protein L1 in Hansenula polymorpha

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Abstract

Two versions of an optimized gene that encodes human papilloma virus type 16 major protein L1 were designed according to the codon usage frequency of Pichia pastoris. Y16 was highly expressed in both P. pastoris and Hansenula polymorpha. M16 expression was as efficient as that of Y16 in P. pastoris, but merely detectable in H. polymorpha even though transcription levels of M16 and Y16 were similar. H. polymorpha had a unique codon usage frequency that contains many more rare codons than Saccharomyces cerevisiae or P. pastoris. These findings indicate that even codon-optimized genes that are expressed well in S. cerevisiae and P. pastoris may be inefficiently expressed in H. polymorpha; thus rare codons must be avoided when universal optimized gene versions are designed to facilitate expression in a variety of yeast expression systems, especially H. polymorpha is involved.

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Acknowledgments

This work was supported by Applied Basic Research Projects of Yunnan Province, China from Yunnan Provincial Science and Technology Department (2013FZ137 and 2010ZC232) and Fundamental Research Funds for the Central Universities of China (2012N08).

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Correspondence to Yanbing Ma.

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Liu, C., Yang, X., Yao, Y. et al. Diverse expression levels of two codon-optimized genes that encode human papilloma virus type 16 major protein L1 in Hansenula polymorpha . Biotechnol Lett 36, 937–945 (2014). https://doi.org/10.1007/s10529-014-1455-z

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