Abstract
An aerobic denitrifier was isolated from activated sludge and the isolate possessed an average removal rate of 5.7 mg NO3 −-N l−1 h−1 without accumulation of NO2 −-N (less than 2.1 mg l−1). The average removal efficiency of nitrate was 93.7 % in 24 h, when the dissolved oxygen (DO) concentrations ranged from 3.2 to 17.5 mg l−1. The activity of both nap (periplasmic nitrate reductase) and nir (nitrite reductase), whose corresponding genes (napA and nirS) were amplified by touchdown PCR, could be responsible for the tolerance of DO concentrations. Other three genes relating to narG, norB and nosZ were noted to involve in isolate strain.
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Acknowledgments
This research was financially supported by the open fund of State Key Lab of Urban Water Resources and Environment (Nos. QA200810, QAK201014) and the National Natural Science Foundation of China (Nos. 51008239, 51208397, 51378400).
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Supplementary Fig. 1
Phylogenetic tree based on partial 16S rRNA sequences
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Ji, B., Wang, H. & Yang, K. Tolerance of an aerobic denitrifier (Pseudomonas stutzeri) to high O2 concentrations. Biotechnol Lett 36, 719–722 (2014). https://doi.org/10.1007/s10529-013-1417-x
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DOI: https://doi.org/10.1007/s10529-013-1417-x