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Comparison of extraction phases for a two-phase culture of a recombinant E. coli producing retinoids

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Abstract

To prevent degradation of intracellular retinoids through in situ extraction from the cells, a two-phase culture system was performed. Several organic solvents, including n-alkanes, mineral oils and cosmetic raw materials, were applied as the extraction phase. Of the n-alkanes, n-decane had the highest retinoid production as 134 mg/l after 72 h. For mineral oil, light and heavy mineral oil gave retinoid productions of 158 and 174 mg/l after 96 h, respectively. Of other materials, isopropyl myristate gave the highest retinoid production of 181 mg/l. These results indicate that many types of oils can be applied for retinoid production, and optimization of the in situ extraction process will lead to further improve of economical production for the industrial purpose.

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References

  • Aono R (1998) Improvement of organic solvent tolerance level of Escherichia coli by overexpression of stress-responsive genes. Extremophiles 2:239–248

    Article  PubMed  CAS  Google Scholar 

  • Hejazi MA, Wijffels RH (2003) Effect of light intensity on beta-carotene production and extraction by Dunaliella salina in two-phase bioreactors. Biomol Eng 20:171–175

    Article  PubMed  CAS  Google Scholar 

  • Inoue A, Horikoshi K (1989) A pseudomonas thrives in high-concentrations of toluene. Nature 338:264–266

    Article  CAS  Google Scholar 

  • Jang HJ, Yoon SH, Ryu HK, Kim JH, Wang CL, Kim JY, Oh DK, Kim SW (2011) Retinoid production using metabolically engineered Escherichia coli with a two-phase culture system. Microb Cell Fact 10:59

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  • Kim SK, Karadeniz F (2012) Biological importance and applications of squalene and squalane. Adv Food Nutr Res 65:223–233

    Article  PubMed  Google Scholar 

  • Kleinegris DM, Janssen M, Brandenburg WA, Wijffels RH (2011) Continuous production of carotenoids from Dunaliella salina. Enzyme Microb Technol 48:253–259

    Article  PubMed  CAS  Google Scholar 

  • Kuo CH, Chen HH, Chen JH, Liu YC, Shieh CJ (2012) High yield of wax ester synthesized from cetyl alcohol and octanoic acid by Lipozyme RMIM and Novozym 435. Int J Mol Sci 13:11694–11704

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  • Leon R, Vila M, Hernanz D, Vilchez C (2005) Production of phytoene by herbicide-treated microalgae Dunaliella bardawil in two-phase systems. Biotechnol Bioeng 92:695–701

    Article  PubMed  CAS  Google Scholar 

  • Martinez A, Bradley AS, Waldbauer JR, Summons RE, DeLong EF (2007) Proteorhodopsin photosystem gene expression enables photophosphorylation in a heterologous host. Proc Natl Acad Sci USA 104:5590–5595

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  • Mercier Chabardes (1994) Organometallic chemistry in industrial vitamin A and vitamin E synthesis. Pure Appl Chem 66:1509–1518

    Article  CAS  Google Scholar 

  • Nash JF, Gettings SD, Diembeck W, Chudowski M, Kraus AL (1996) A toxicological review of topical exposure to white mineral oils. Food Chem Toxicol 34:213–225

    Article  PubMed  CAS  Google Scholar 

  • Nicolaou SA, Gaida SM, Papoutsakis ET (2010) A comparative view of metabolite and substrate stress and tolerance in microbial bioprocessing: from biofuels and chemicals, to biocatalysis and bioremediation. Metab Eng 12:307–331

    Article  PubMed  CAS  Google Scholar 

  • Ramos JL, Duque E, Gallegos MT, Godoy P, Ramos-Gonzalez MI, Rojas A, Teran W, Segura A (2002) Mechanisms of solvent tolerance in gram-negative bacteria. Annu Rev Microbiol 56:743–768

    Article  PubMed  CAS  Google Scholar 

  • Rawlings AV, Lombard KJ (2012) A review on the extensive skin benefits of mineral oil. Int J Cosmet Sci 34:511–518

    Article  PubMed  CAS  Google Scholar 

  • Sabehi G, Loy A, Jung KH, Partha R, Spudich JL, Isaacson T, Hirschberg J, Wagner M, Beja O (2005) New insights into metabolic properties of marine bacteria encoding proteorhodopsins. PLoS Biol 3:e273

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  • Sorg O, Antille C, Kaya G, Saurat JH (2006) Retinoids in cosmeceuticals. Dermatol Ther 19:289–296

    Article  PubMed  Google Scholar 

  • Traul KA, Driedger A, Ingle DL, Nakhasi D (2000) Review of the toxicologic properties of medium-chain triglycerides. Food Chem Toxicol 38:79–98

    Article  PubMed  CAS  Google Scholar 

  • Wang C, Yoon SH, Shah AA, Chung YR, Kim JY, Choi ES, Keasling JD, Kim SW (2010) Farnesol production from Escherichia coli by harnessing the exogenous mevalonate pathway. Biotechnol Bioeng 107:421–429

    Article  PubMed  CAS  Google Scholar 

  • Yoon KW, Doo EH, Kim SW, Park JB (2008) In situ recovery of lycopene during biosynthesis with recombinant Escherichia coli. J Biotechnol 135:291–294

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by a grant (NRF-2010-C1AAA001-0029084) from the National Research Foundation, the Intelligent Synthetic Biology Center of Global Frontier Project funded by the MEST (2011-0031964), and a grant from the Next-Generation BioGreen 21 Program (SSAC, Grant#: PJ009522003), RDA, Korea.

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Authors and Affiliations

  1. Division of Applied Life Science (BK21 Program), PMBBRC, Gyeongsang National University, Jinju, 660-701, Korea

    Hui-Jeong Jang, Bo-Kyung Ha, Sang-Hwal Yoon & Seon-Won Kim

  2. Department of Applied Chemistry, Hanyang University, Ansan, Gyeonggi-do, 426-791, Korea

    Jin-Woong Kim

  3. Department of Food Science & Technology, Kyungsung University, Pusan, 608-736, Korea

    Kyung-Hwa Jung

  4. Department of Life Sciences, Imperial College London, South Kensington Campus, London, SW7 2AZ, UK

    Jiyoon Ahn

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  1. Hui-Jeong Jang
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  2. Bo-Kyung Ha
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  4. Kyung-Hwa Jung
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Correspondence to Sang-Hwal Yoon.

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Hui-Jeong Jang and Bo-Kyung Ha have contributed equally to this work

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Jang, HJ., Ha, BK., Kim, JW. et al. Comparison of extraction phases for a two-phase culture of a recombinant E. coli producing retinoids. Biotechnol Lett 36, 497–505 (2014). https://doi.org/10.1007/s10529-013-1385-1

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  • DOI: https://doi.org/10.1007/s10529-013-1385-1

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