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Engineered Salmonella typhimurium expressing E7 fusion protein, derived from human papillomavirus, inhibits tumor growth in cervical tumor-bearing mice

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Abstract

The tumor-suppressing effects of SipB160/HPV16 E7 fusion protein, derived from human papillomavirus, and expressed in Salmonella enterica serovar typhimurium, were evaluated in a cervical cancer model. The expressed E7 protein resulted in efficacious cytotoxicity and tumor growth retardation in TC-1 cervical cancer cells. In addition, in mice bearing TC-1 tumors, live cells of Salmonella expressing HPV16 E7 were administered orally and induced immune responses through interferon-gamma and tumor necrosis factor-alpha cytokine secretion and also suppressed tumor growth (45 %) and prolonged survival (70 %) compared with the control group. These results suggested that the SipB160/HPV16 E7 fusion protein may be a candidate cancer therapeutic agent.

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Acknowledgments

We thank Im Hana for providing technical assistance. This study was supported by a grant of the KOREA UNIVERSITY, This study was also supported by NRF Fund (2013R1A1A2005567) from Government of KOREA.

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  1. School of Life Sciences and Biotechnology, Korea University, Seoul, 136-705, Republic of Korea

    Wonsuck Yoon, Ji Hyeon Choi, Sinyeon Kim & Yong Keun Park

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Correspondence to Yong Keun Park.

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Yoon, W., Choi, J.H., Kim, S. et al. Engineered Salmonella typhimurium expressing E7 fusion protein, derived from human papillomavirus, inhibits tumor growth in cervical tumor-bearing mice. Biotechnol Lett 36, 349–356 (2014). https://doi.org/10.1007/s10529-013-1370-8

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