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Two-helper-strain co-culture system: a novel method for enhancement of 2-keto-l-gulonic acid production

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Abstract

A novel two-helper-strain co-culture system (TSCS) was developed to enhance 2-keto-l-gulonic acid (2-KLG) productivity for vitamin C production. Bacillus megaterium and B. cereus (with a seeding culture ratio of 1:3, v/v), used as helper strains, increased the 2-KLG yield using Ketogulonigenium vulgare compared to the conventional one-helper-strain (either B. cereus or B. megaterium) co-culture system (OSCS). After 45 h cultivation, 2-KLG concentration in the TSCS (69 g l−1) increased by 8.9 and 7 % over that of the OSCS (B. cereus: 63.4 g l−1; B. megaterium: 64.5 g l−1). The fermentation period of TSCS was 4 h shorter than that of OSCS (B. cereus). The increased cell numbers of K. vulgare stimulated by the two helper strains possibly explain the enhanced 2-KLG yield. The results imply that TSCS is a viable method for enhancing industrial production of 2-KLG.

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Acknowledgments

This research was financially supported by the Program of National Key New Drug Creation of China (No. 2011ZX09203-001-14).

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Authors and Affiliations

  1. Institute of Applied Ecology, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang, 110016, China

    Mandlaa, Weichao Yang, Litao Han & Hui Xu

  2. University of Chinese Academy of Sciences, Beijing, 100039, China

    Mandlaa & Zhenyu Wang

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  1. Mandlaa
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  2. Weichao Yang
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  3. Litao Han
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  4. Zhenyu Wang
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  5. Hui Xu
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Corresponding author

Correspondence to Hui Xu.

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Mandlaa and Weichao Yang have contributed equally to this work.

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Mandlaa, Yang, W., Han, L. et al. Two-helper-strain co-culture system: a novel method for enhancement of 2-keto-l-gulonic acid production. Biotechnol Lett 35, 1853–1857 (2013). https://doi.org/10.1007/s10529-013-1292-5

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  • DOI: https://doi.org/10.1007/s10529-013-1292-5

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