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A mutation in the Xanthomonas oryzae pv. oryzae wxoD gene affects xanthan production and chemotaxis

Biotechnology Letters volume 35, pages 1913–1918 (2013)Cite this article

Abstract

Xanthomonas oryzae pv. oryzae causes bacterial blight in rice (Oryza sativa L.). The effect of a mutation in the wxoD gene, that encodes a putative O-antigen acetylase, on xanthan production as well as bacterial chemotaxis was investigated. The mutation increased xanthan production by 52 %. The mutant strain was non-motile on semi-solid agar swarm plates. In addition, several genes involved in chemotaxis, including the cheW, cheV, cheR, and cheD genes, were down-regulated by a mutation in the wxoD gene. Thus, the mutation in the wxoD gene affects xanthan production as well as bacterial chemotaxis. However, the wxoD gene is not essential for the virulence of X. oryzae.

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Acknowledgments

This work was supported by Konkuk University.

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  1. Department of Biomedical Chemistry, Konkuk University, Chung-Ju, 380-701, Republic of Korea

    Jae-Young Nam, Hong-Il Kim, Chang-Soo Lee & Young-Jin Park

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  1. Jae-Young Nam
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  2. Hong-Il Kim
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  3. Chang-Soo Lee
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Correspondence to Young-Jin Park.

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Nam, JY., Kim, HI., Lee, CS. et al. A mutation in the Xanthomonas oryzae pv. oryzae wxoD gene affects xanthan production and chemotaxis. Biotechnol Lett 35, 1913–1918 (2013). https://doi.org/10.1007/s10529-013-1285-4

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  • DOI: https://doi.org/10.1007/s10529-013-1285-4

Keywords

  • Chemotaxis
  • Mutation
  • O-antigen acetylase
  • wxoD
  • Xanthan
  • Xanthomonas oryzae pv. oryzae