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Mechanical stimulation and the presence of neighboring cells greatly affect migration of human mesenchymal stem cells

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Abstract

There are few studies regarding the effects of mechanical stimulation on cell migration although biochemical factors have been widely studied. We have investigated the effects of intermittent hydrostatic pressure (IHP) on mesenchymal stem cell migration with or without neighboring endothelial cells (EC). IHP promoted MSCs migration and the neighboring ECs helped with this. However, when IHP was applied to MSCs cultured with ECs, the opposite effect was observed. The concentration of stromal-derived factor-1 culture in medium was measured to explain the obtained results. SDF-1 concentration increased as IHP increased when MSCs were cultured alone. However, it decreased as IHP increased when MSCs and ECs were co-cultured. These results indicate that the mechanical environment should be considered when studying the migration of a cell type along with its biochemical environment.

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Acknowledgments

This work was supported by the Grants of Technology Innovation Program (10038667, Ministry of Knowledge Economy, ROK) and Priority Research Centers Program (2010-0020224, the Ministry of Education, Science and Technology).

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Authors and Affiliations

  1. Department of Biomedical Engineering, Inje University, 607 Eubang-Dong, Gimhae, Gyeongnam, 621-749, South Korea

    Seon Yeon Kim, So Hee Park, Yun Gyeong Kang, Kang Jin Jeon, Jin-Sook Hyun, Min-Jae Oh & Jung-Woog Shin

  2. Cardiovascular and Metabolic Disease Center, Inje University, Busan, South Korea

    Ji Won Shin & Jung-Woog Shin

  3. Institute of Aged Life Redesign/UHRC, Inje University, Rm #309, BLDG-A, 607 Eubang-Dong, Gimhae, Gyeongnam, 621-749, South Korea

    Jung-Woog Shin

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  1. Seon Yeon Kim
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  2. So Hee Park
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  3. Ji Won Shin
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  4. Yun Gyeong Kang
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  5. Kang Jin Jeon
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  7. Min-Jae Oh
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Correspondence to Jung-Woog Shin.

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Kim, S.Y., Park, S.H., Shin, J.W. et al. Mechanical stimulation and the presence of neighboring cells greatly affect migration of human mesenchymal stem cells. Biotechnol Lett 35, 1817–1822 (2013). https://doi.org/10.1007/s10529-013-1270-y

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  • DOI: https://doi.org/10.1007/s10529-013-1270-y

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