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MiR-424 regulates monocytic differentiation of human leukemia U937 cells by directly targeting CDX2

Biotechnology Letters volume 35, pages 1799–1806 (2013)Cite this article

Abstract

MiR-424 plays an important role via promoting the monocytic differentiation in many human leukemia cell lines. Here, we report that miR-424 decreased miR-125b expression to 36 % by directly targeting caudal type homeobox 2. However, miR-424 also decreased expression of Fes, PU.1 and colony-stimulating factor receptor (MCSFR). As Fes, PU.1 and MCSFR were down-regulated by over-expression of miR-125b (unpublished work), a similar effect of miR-424 and Fes siRNA on CD64, Egr-1, Egr-2 and CEBPA indicates that Fes may be an important downstream target of miR-424. We hypothesize that miR-424 promotes monocytic differentiation by regulating other critical factors and miR-424 has high affinity for these factors. For the first time, the molecular mechanism of miR-424 during monocytic differentiation of U937 cells has been elucidated in this study.

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Acknowledgments

We thank Prof. Qinglong Guo for providing the cell lines used in this study, M.D. Xuepeng Deng and M.D. Jiajie Tu for helpful comments on the manuscript and the members of the Tao Xi lab for helpful discussion.

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Affiliations

  1. School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, People’s Republic of China

    Xiao Shen, Jinhang Hu, Le Guo, Yingying Xing & Tao Xi

  2. Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University, Nanjing, 210009, People’s Republic of China

    Xiao Shen, Jinhang Hu, Le Guo, Yingying Xing & Tao Xi

  3. Jiangsu Cancer Hospital, Nanjing, 210009, People’s Republic of China

    Jinhai Tang

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  1. Xiao Shen
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  2. Jinhai Tang
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  3. Jinhang Hu
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  4. Le Guo
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  5. Yingying Xing
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  6. Tao Xi
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Correspondence to Tao Xi.

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Shen, X., Tang, J., Hu, J. et al. MiR-424 regulates monocytic differentiation of human leukemia U937 cells by directly targeting CDX2. Biotechnol Lett 35, 1799–1806 (2013). https://doi.org/10.1007/s10529-013-1264-9

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  • DOI: https://doi.org/10.1007/s10529-013-1264-9

Keywords

  • CDX2
  • Fes
  • Human leukemia cells (U937)
  • miR-125b
  • miR-424
  • Monocytic differentiation