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Improving the thermostability of Escherichia coli phytase, appA, by enhancement of glycosylation

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Abstract

A codon-optimized Escherichia coli appA phytase gene was synthesized and expressed in Pichia pastoris. Two residue substitutions (Q258N, Q349N) were sequentially introduced to enhance its glycosylation activity. Secretion of appA-Q258N/Q349N was approx. 0.3 mg ml−1 and enzyme activity reached 1,030 U ml−1. Purified appA-Q258N/Q349N had a specific activity of 3,137 U mg−1 with an MW of approx. 53 kDa. Compared with appA-WT, appA-Q258N/Q349N showed over 40 % enhancement in thermostability (85 °C for 10 min) and 4–5 °C increases in the melting temperatures (Tm). The Km and Kcat of appA-Q258N/Q349N were 0.43 mM and 3,058 s−1, respectively, which are similar with that of appA-WT. The mutant appA-Q258N/Q349N obtained in this study could be used for the large-scale commercial production of phytase.

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Acknowledgements

This work was supported by Grants from the “National Natural Science Foundation of China” (No. 31071924, 31272100), the “Natural Science Foundation of Shanxi Province” (2010011040-1) and the “Shanxi Scholarship Council of China”.

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Authors and Affiliations

  1. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006, People’s Republic of China

    Ming-Ze Yao, Xi Wang, Wei Wang, Yue-Jun Fu & Ai-Hua Liang

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  1. Ming-Ze Yao
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  2. Xi Wang
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  3. Wei Wang
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  5. Ai-Hua Liang
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Correspondence to Ai-Hua Liang.

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Yao, MZ., Wang, X., Wang, W. et al. Improving the thermostability of Escherichia coli phytase, appA, by enhancement of glycosylation. Biotechnol Lett 35, 1669–1676 (2013). https://doi.org/10.1007/s10529-013-1255-x

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  • DOI: https://doi.org/10.1007/s10529-013-1255-x

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