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Degradation of a xenobiotic textile dye, Disperse Brown 118, by Brevibacillus laterosporus

Abstract

The toxic textile dye, Disperse Brown 118, was degraded by Brevibacillus laterosporus. 96 % decolorization was achieved within 48 h at pH 7, 40 °C at 50 mg dye l−1 accompanied by significant increases in the activities of veratryl alcohol oxidase, tyrosinase and NADH-DCIP reductase. HPTLC and FT-IR spectroscopy confirmed biodegradation after dye decolorization. As identified by GC–MS, biodegradation products of Disperse Brown 118 were N-carbamoyl-2-[(8-chloroquinazolin-4-yl)oxy] acetamide and N-carbamoyl-2-(quinazolin-4-yloxy)acetamide which were much less toxic than parent dye as evidenced by phytotoxicity tests.

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References

  • Chang JS, Lin YC (2001) Decolorization kinetics of recombinant E coli strain harboring azo dye decolorization determinants for Rhodococcus sp. Biotechnol Lett 23:631–636

    Article  CAS  Google Scholar 

  • Constapel M, Schellentra¨er M, Marzinkowski JM, Gab S (2009) Degradation of reactive dyes in wastewater from the textile industry by ozone: analysis of the products by accurate masses. Water Res 43:733–743

    PubMed  Article  CAS  Google Scholar 

  • Figueroa S, Vázqueza L, Alvarez-Gallegos A (2009) Decolorizing textile wastewater with Fenton’s reagent lector generated with a solar photovoltaic cell. Water Res 43:283–294

    PubMed  Article  CAS  Google Scholar 

  • Jadhav UU, Dawkar VV, Ghodake GS, Govindwar SP (2008) Biodegradation of Direct Red 5B a textile dye by newly isolated Comamonas sp. UVS. J Hazard Mater 158:507–516

    PubMed  Article  CAS  Google Scholar 

  • Jadhav UU, Dawkar VV, Tamboli DP, Govindwar SP (2009) Purification and characterization of veratryl alcohol oxidase from Commamonas sp. UVS and its role in decolorization of textile dyes. Biotechnol Bioprocess Eng 14:369–376

    Article  CAS  Google Scholar 

  • Kabil A (2000) Chlorotriazine reactive Azo red 120 textile dye induces micronuclei in fish. Ecotoxicol Environ Saf 47:149–155

    Article  Google Scholar 

  • Kurade MB, Waghmode TR, Kagalkar AN, Govindwar SP (2012a) Decolorization of textile industry effluent containing disperse dye Scarlet RR by a newly developed bacterial-yeast consortium BL-GG. Chem Eng J 184:33–41

    Article  CAS  Google Scholar 

  • Kurade MB, Waghmode TR, Tamboli DP, Govindwar SP (2012b) Differential catalytic action of Brevibacillus laterosporus MTCC 2298 on two dissimilar azo dyes Remazol red and Rubine GFL. J Basic Microbiol 53:136–146

    PubMed  Article  Google Scholar 

  • Parshetti GK, Telke AA, Kalyani DC, Govindwar SP (2009) Decolorization and detoxification of sulfonated azo dye methyl orange by Kocuria rosea MTCC 1532. J Hazard Mater 176:503–509

    PubMed  Article  Google Scholar 

  • Phugare S, Waghmare S, Jadhav J (2011) Purification and characterization of dye degrading of veratryl alcohol oxidase from Pseudomonas aeruginosa strain BCH. World J Microbiol Biotechnol 27:2415–2423

    Article  CAS  Google Scholar 

  • Sumathi S, Manju B (2000) Uptake of reactive textile dyes by Aspergillus foetidus. Enzyme Microbial Technol 27:347–355

    Article  CAS  Google Scholar 

  • Waghmode TR, Kurade MB, Lade HS, Govindwar SP (2012a) Effect of temperature on the decolorization of Rubine GFL by developed microbial consortium GG-BL in sequential aerobic/microaerophilic process. Appl Biochem Biotechnol 167:1578–1594

    PubMed  Article  CAS  Google Scholar 

  • Waghmode TR, Kurade MB, Lade HS, Govindwar SP (2012b) Biodegradation of Rubine GFL by Galactomyces geotrichum MTCC 1360 and subsequent toxicological analysis by using cytotoxicity, genotoxicity and oxidative stress studies. Microbiology 158:2344–2352

    PubMed  Article  CAS  Google Scholar 

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Acknowledgments

M. B. Kurade and T. R. Waghmode wishes to thank Department of Biotechnology, New Delhi, India for providing junior research fellowship.

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Correspondence to Sanjay P. Govindwar.

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Kurade, M.B., Waghmode, T.R., Kabra, A.N. et al. Degradation of a xenobiotic textile dye, Disperse Brown 118, by Brevibacillus laterosporus . Biotechnol Lett 35, 1593–1598 (2013). https://doi.org/10.1007/s10529-013-1253-z

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  • DOI: https://doi.org/10.1007/s10529-013-1253-z

Keywords

  • Biodegradation
  • Bioremediation
  • Biotransformation
  • Detoxification
  • Phytotoxicity