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Enhancing the supply of oxaloacetate for l-glutamate production by pyc overexpression in different Corynebacterium glutamicum

Biotechnology Letters volume 35, pages 943–950 (2013)Cite this article

Abstract

During l-glutamate production, phosphoenolpyruvate carboxylase and pyruvate carboxylase (PCx) play important roles in supplying oxaloacetate to the tricarboxylic acid cycle. To explore the significance of PCx for l-glutamate overproduction, the pyc gene encoding PCx was amplified in Corynebacterium glutamicum GDK-9 triggered by biotin limitation and CN1021 triggered by a temperature shock, respectively. In the fed-batch cultures, GDK-9pXMJ19pyc exhibited 7.4 % lower l-alanine excretion and no improved l-glutamate production. In contrast, CN1021pXMJ19pyc finally exhibited 13 % lower l-alanine excretion and identical l-glutamate production, however, 8.5 % higher l-glutamate production was detected during a short period of the fermentation. It was indicated that pyc overexpression in l-glutamate producer strains, especially CN1021, increased the supply of oxaloacetate for l-glutamate synthesis and decreased byproduct excretion at the pyruvate node.

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Acknowledgments

We gratefully acknowledge the support from Jing Wang. This work was supported by National Key Technology Research and Development Program of the Ministry of Science and Technology (2011BAC11B03), National High Technology Research and Development Program (SS2013AA100106) and Program for Changjiang Scholars and Innovative Research Team in University (IRT 1166).

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Affiliations

  1. National and Local United Engineering Lab of Metabolic Control Fermentation Technology, Tianjin University of Science and Technology, Tianjin, 300457, China

    Xuan Guo, Jing Wang, Xixian Xie, Qingyang Xu, Chenglin Zhang & Ning Chen

  2. Tianjin Engineering Lab of Efficient and Green Amino Acid Manufacture, Tianjin University of Science and Technology, Tianjin, 300457, China

    Xuan Guo, Jing Wang, Xixian Xie, Qingyang Xu, Chenglin Zhang & Ning Chen

  3. Key Laboratory of Industrial Microbiology of Education Ministry, Tianjin University of Science and Technology, Tianjin, 300457, China

    Xuan Guo, Jing Wang, Xixian Xie, Qingyang Xu, Chenglin Zhang & Ning Chen

  4. Metabolic Engineering Laboratory, College of Biotechnology, Tianjin University of Science and Technology, No. 29, 13 Main Street, Tianjin Economic and Technological Development Area, Tianjin, 300457, People’s Republic of China

    Ning Chen

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  1. Xuan Guo
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  2. Jing Wang
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  3. Xixian Xie
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  4. Qingyang Xu
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  5. Chenglin Zhang
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  6. Ning Chen
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Correspondence to Ning Chen.

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Guo, X., Wang, J., Xie, X. et al. Enhancing the supply of oxaloacetate for l-glutamate production by pyc overexpression in different Corynebacterium glutamicum . Biotechnol Lett 35, 943–950 (2013). https://doi.org/10.1007/s10529-013-1241-3

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  • DOI: https://doi.org/10.1007/s10529-013-1241-3

Keywords

  • Biotin
  • Byproduct
  • Corynebacterium glutamicum
  • l-Glutamate
  • Pyruvate carboxylase