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Identification of the proteins in green cotton fiber using a proteomics-based approach

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Abstract

The proteome of 21 DPA (days post-anthesis) of green cotton fiber was analyzed using two-dimensional gel electrophoresis to yield the first reference proteome map. Of 220 individual spots that were excised and analyzed by MALDI-TOF/TOF MS, 156 were identified and cataloged according to their functions. Twelve classes of proteins were identified. Many of these proteins were related to carbohydrate metabolism and energy production. Other notable functional classes included oxidoreductase, cell wall-related and cytoskeleton proteins. This study gives a snapshot of the proteome in 21 DPA green cotton fiber and advances our understanding of molecular mechanisms related to pigment biosynthesis in green cotton fiber.

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Acknowledgments

This work was funded by the National Basic Research Priorities Program (Grant No: 2007CD116210). We are grateful to Dr. Chun-Lin Yang and Hai-Yun Wang for their technical assistance with 2-DE and gels analyses. We also thank Mr. Ming-Liang Liu, Dr. Jian-Lan Yu and Ji Luo (AB Sciex) for their kind assistance in MS/MS analysis and database searching of proteins.

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Authors and Affiliations

  1. The Key Laboratory of Oasis Eco-agriculture, College of Agriculture, Shihezi University, Shihezi, 832003, Xinjiang, People’s Republic of China

    Yan-Jun Li, Ya-Qin Wang, Yong-Chang Liu, Xin-Yu Zhang & Jie Sun

  2. State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of China

    Fu-Xin Wang

  3. College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, 310036, Zhejiang, People’s Republic of China

    Yu-Qiang Sun

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  1. Yan-Jun Li
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  2. Fu-Xin Wang
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  3. Ya-Qin Wang
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  4. Yong-Chang Liu
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  5. Xin-Yu Zhang
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  6. Yu-Qiang Sun
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  7. Jie Sun
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Corresponding author

Correspondence to Jie Sun.

Additional information

Yan-Jun Li and Fu-Xin Wang contributed equally to this study.

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Li, YJ., Wang, FX., Wang, YQ. et al. Identification of the proteins in green cotton fiber using a proteomics-based approach. Biotechnol Lett 35, 1519–1523 (2013). https://doi.org/10.1007/s10529-013-1227-1

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  • DOI: https://doi.org/10.1007/s10529-013-1227-1

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