Abstract
A 1,697-bp cDNA sequence, designated as PsG6PDH, was amplified from Populus suaveolens. Multiple sequence alignment and phylogenetic analysis indicated that PsG6PDH encodes a cytosolic G6PDH isoform, with Southern blot analysis demonstrating that the gene is single or low copy in Populus. Transgenic tobacco plants over-expressing PsG6PDH exhibited enhanced cold tolerance. In both transgenic and wild-type (WT) tobacco plants, cold stress increased leaf malondialdehyde (MDA) content, electrolyte leakage (EL), and peroxide (POD) and superoxide dismutase (SOD) activities; relative to WT, however, transgenic lines had lower MDA content and EL and higher SOD and POD activities. In addition, PsG6PDH activated the expression of stress-related genes, including NtERD10b, NtERD10c, and NtSOD, in tobacco plants. Our results provide evidence regarding PsG6PDH regulatory function in plants during low temperature stress.
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References
Asai S, Yoshioka M, Nomura H, Tone C, Nakajima K, Nakane E, Doke N, Yoshioka H (2011) A plastidic glucose-6-phosphate dehydrogenase is responsible for hypersensitive response cell death and reactive oxygen species production. J Gen Plant Pathol 77:152–162
Cardi M, Chibani K, Cafasso D, Rouhier N, Jacquot JP, Esposito S (2011) Abscisic acid effects on activity and expression of barley (Hordeum vulgare) plastidial glucose-6-phosphate dehydrogenase. J Exp Bot 62:4013–4023
Dennis DT, Huang Y, Negm FB (1997) Glycolysis, the pentose phosphate pathway and anaerobic respiration. In: Dennis DT, Huang Y (eds) Plant metabolism. Longman Press, Harlow, pp 105–123
Esposito S, Guerriero G, Vona V, Martino RV, Carfagna S, Rigano C (2005) Glutamate synthase activities and protein changes in relation to nitrogen nutrition in barley: the dependence on different plastidial glucose-6P dehydrogenase isoforms. J Exp Bot 56:55–64
Gapper C, Dolan L (2006) Control of plant development by reactive oxygen species. Plant Physiol 141:341–345
Honjoh K, Machida T, Hagisako T, Suga K, Yonekura M, Shimizu H, Ohashi N, Miyamoto T, Hatano S, Iio M (2007) Molecular cloning and characterization of a cDNA for low-temperature inducible cytosolic glucose 6-phosphate dehydrogenase gene from Chlorella vulgaris and expression of the gene in Saccharomyces cerevisiae. Plant Sci 172:649–658
Knight JS, Emes MJ, Debnam PM (2001) Isolation and characterisation of a full-length genomic clone encoding a plastidic glucose 6-phosphate dehydrogenase from Nicotiana tabacum. Planta 212:499–507
Lin SZ, Zhang ZY, Liu WF, Lin YZ, Zhang Q, Zhu BQ (2005) Role of glucose-6-phosphate dehydrogenase in freezing-induced freezing resistance of Populus suaveolens. J Plant Physiol Mol Biol 31:34–40
Lin YZ, Zheng HQ, Zhang Q, Liu CX, Zhang ZY (2013) Functional profiling of EcaICE1 transcription factor gene from Eucalyptus camaldulensis involved in cold response in tobacco plants. J Plant Biochem Biotechnol. doi:10.1007/s13562-013-0192-z
Ludek SM, Lenka B (1999) Changes in activity of glucose-6-phosphate and 6-phosphogluconate dehydrogenase isozymes upon potato virus Y infection in tobacco leaf tissues and protoplasts. Plant Physiol Biochem 37:195–201
Nemoto Y, Sasakuma T (2000) Specific expression of glucose-6-phosphate dehydrogenase (G6PDH) gene by salt stress in wheat (Triticum aestivum L.). Plant Sci 158:53–60
Scharte J, Schön H, Tjaden Z, Weis E, vonSchaewen A (2009) Isoenzyme replacement of glucose-6-phosphate dehydrogenase in the cytosol improves stress tolerance in plants. PNAS USA 106:8061–8066
Slaski JJ, Zhang G, Basu U, Stephens JL, Taylor GL (1996) Aluminium resistance in wheat (Triticum aesticum) is associated with rapid, Al-induced changes in activities of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase in root apices. Physiol Plant 98:477–484
Wakao S, Benning C (2005) Genome-wide analysis of glucose-6-phosphate dehydrogenases in Arabidopsis. Plant J 41:243–256
Acknowledgments
This work was supported by Nation Natural Science Foundation of China (30271093), Foundation for Distinguished Young Talents in Higher Education of Guangdong, China (LYM10040), and Key Laboratory of Biomass Energy of Guangdong Higher Education Institutes, South China Agricultural University, China (BOP2012-7). We thank Professor Harry Wu from CSIRO Plant Industry in Australia and Dr. Huiquan Zheng from Guangdong Academy of Forestry in China for paper revision.
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Lin, Y., Lin, S., Guo, H. et al. Functional analysis of PsG6PDH, a cytosolic glucose-6-phosphate dehydrogenase gene from Populus suaveolens, and its contribution to cold tolerance improvement in tobacco plants. Biotechnol Lett 35, 1509–1518 (2013). https://doi.org/10.1007/s10529-013-1226-2
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DOI: https://doi.org/10.1007/s10529-013-1226-2