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Functional analyses of recombinant mouse hepcidin-1 in cell culture and animal model

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Hepcidin is a peptide hormone that plays an important role in iron metabolism. We have produced a recombinant mouse hepcidin-1 by using baculovirus expression system. Its expression yield was 25 μg/ml when cell culture media were supplemented with a protease inhibitor cocktail. The recombinant mouse hepcidin-1 and synthetic human hepcidin-25 had similar effects on reducing ferroportin expression in J774A cell line and in peritoneal macrophages. However, synthetic human hepcidin-25 was more efficient than recombinant mouse hepcidin-1 in reducing iron concentration in blood circulation (p < 0.01).

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This study has been funded by the Golestan University of Medical Sciences and Isfahan University of Medical Sciences.

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Correspondence to Yaghoub Yazdani.

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Yazdani, Y., Keyhanvar, N., Kalhor, H.R. et al. Functional analyses of recombinant mouse hepcidin-1 in cell culture and animal model. Biotechnol Lett 35, 1191–1197 (2013).

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