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Extraction of ginsenosides from fresh ginseng roots (Panax ginseng C.A. Meyer) using commercial enzymes and high hydrostatic pressure

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Abstract

A combination of high hydrostatic pressure (HHP) and enzymatic hydrolysis (HHP-EH) was applied for the extraction of ginsenosides from fresh ginseng roots (Panax ginseng C.A. Myer). The highest yield of ginsenosides was obtained by using a mixture of three enzymes (Celluclast + Termamyl + Viscozyme) along with HHP (100 MPa, at 50 °C for 12 h) in comparison to control samples (no enzymes, atmosphere pressure, P < 0.05). Total ginsenosides increased by 184 % while Rg1 + Rb1 increased by 273 %. Application of these conditions significantly increased total ginsenosides by 49 % and Rg1 + Rb1 by 103 % compared to HHP treatment alone (P < 0.05). The effect of HHP on increased yield of ginsenosides is likely due in part, to acceleration of enzyme activity. Thus HHP-EH significantly improves the extraction of ginsenosides from fresh ginseng roots.

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Acknowledgments

This research was funded by the Food High Pressure Technology Development Project, Korea Food Research Institute, Korea.

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Authors and Affiliations

  1. Faculty of Pharmacy and Pharmaceutical Science, 3-142G Katz Group Centre for Pharmacy & Health Research, University of Alberta, 11361-87 Ave., Edmonton, AB, T6G 2E1, Canada

    Hoon H. Sunwoo

  2. Korea Food Research Institute, Seongnam, Gyeonggi, 463-746, Korea

    Chong-Tai Kim, Do-Yeon Kim, Jin-Soo Maeng & Chang-Won Cho

  3. Department of Food and Nutrition, Bucheon University, Bucheon, Gyeonggi, 420-735, Korea

    Soo-Jeong Lee

Authors
  1. Hoon H. Sunwoo
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  2. Chong-Tai Kim
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  3. Do-Yeon Kim
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  4. Jin-Soo Maeng
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  5. Chang-Won Cho
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  6. Soo-Jeong Lee
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Correspondence to Hoon H. Sunwoo or Chong-Tai Kim.

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Sunwoo, H.H., Kim, CT., Kim, DY. et al. Extraction of ginsenosides from fresh ginseng roots (Panax ginseng C.A. Meyer) using commercial enzymes and high hydrostatic pressure. Biotechnol Lett 35, 1017–1022 (2013). https://doi.org/10.1007/s10529-013-1182-x

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  • DOI: https://doi.org/10.1007/s10529-013-1182-x

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