Skip to main content
Springer Nature Link
Log in

Association of HvLDI with limit dextrinase activity and malt quality in barley

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Limit dextrinase (LD) is a unique de-branching enzyme involved in starch mobilization of barley grains during malting, and closely related to malt quality. Genotypic variation of LD activity is controlled by genetic factors and also affected by environmental conditions. Correlation analysis between LD activity and four malt quality parameters showed that LD activity was positively correlated with diastatic power, Kolbach index and the quality of malt extract, while negatively correlated with viscosity. The structure-based association analysis demonstrated that HvLDI, a gene encoding limit dextrinase inhibitor, was a major determinant of LD activity and malt quality. The single nucleotide polymorphisms associated with LD activity could be used in early generation selection for barley breeding.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+
from $39.99 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Explore related subjects

Discover the latest articles, books and news in related subjects, suggested using machine learning.

References

  • Bradbury PJ, Zhang Z, Kroon DE, Casstevens TM, Ramdoss Y (2007) TASSEL: software for association mapping of complex traits in diverse samples. Bioinformatics 23:2633–2635

    Article  PubMed  CAS  Google Scholar 

  • Fincher GB (1989) Molecular and cellular biology associated with endosperm mobilisation in germinating cereal grains. Annu Rev Plant Physiol Plant Mol Bio 40:305–346

    Article  CAS  Google Scholar 

  • Jin XL, Cai SG, Han Y, Wang J, Wei K, Zhang GP (2011) Genetic variants of HvGlb1 in Tibetan annual wild barley and cultivated barley and their correlation with malt quality. J Cereal Sci 53:149–153

    Article  CAS  Google Scholar 

  • Kristensen M, Planchot FLV, Svendsen I, Leah R, Svensson B (1999) Isolation and characterization of the gene encoding the starch debranching enzyme limit dextrinase from germinating barley. Biochim Biophys Acta 143:538–546

    Article  Google Scholar 

  • MacGregor AW, Macri LJ, Schroeder SW, Bazin SL (1994) Purification and characterisation of limit dextrinase inhibitors from barley. J Cereal Sci 20:33–41

    Article  CAS  Google Scholar 

  • MacGregor AW, Bazin SL, Schroeder SW (2002) Effect of starch hydrolysis products on the determination of limit dextrinase and limit dextrinase inhibitors in barley and malt. J Cereal Sci 35:17–28

    Article  CAS  Google Scholar 

  • McCafferty CA, Perch-Nielsen N, Bryce JH (2000) Effects of aerobic and anaerobic germination on the debranching enzyme, limit dextrinase, in barley malt. J Am Soc Brew Chem 58:47–50

    CAS  Google Scholar 

  • McCleary BV (1992) Measurement of the content of limit dextrinase in cereal flours. Carbohydr Res 227:257–268

    Article  CAS  Google Scholar 

  • Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959

    PubMed  CAS  Google Scholar 

  • Sissons MJ, Taylor M, Proudlove M (1995) Barley malt limit dextrinase: its extraction, heat stability, and activity during malting and mashing. J Am Soc Brew Chem 53:104–110

    CAS  Google Scholar 

  • Stam MR, Danchin EGJ, Rancurel C, Coutinho PM, Henrissat B (2006) Dividing the large glycoside hydrolase family 13 into subfamilies: towards improved functional annotations of α-amylase-related proteins. Protein Eng Des Sel 19:555–562

    Article  PubMed  CAS  Google Scholar 

  • Stenholm K, Home S (1999) A new approach to limit dextrinase and its role in mashing. J Inst Brew 105:205–210

    Article  CAS  Google Scholar 

  • Ullrich SE, Han F, Jones BL (1997) Genetic complexity of the malt extract trait in barley suggested by QTL analysis. J Am Soc Brew Chem 55:1–4

    CAS  Google Scholar 

  • Uzunova M, Eck W, WeiBleder K, Röbbelen G (1995) Mapping the genome of rapeseed (Brassica napus L.). I. Construction of an RFLP linkage map and localization of QTLs for seed glucosinolate content. Theor Appl Genet 90:194–204

    Article  CAS  Google Scholar 

  • Zhang G, Li C (2009) Genetics and improvement of barley malting quality. Zhejiang University Press, Hangzhou and Springer, New York, pp 199–201

    Google Scholar 

Download references

Acknowledgments

This research was supported by National Natural Science Foundation of China (No. 31271656) and Zhejiang Provincial Natural Science Foundation of China (Y3100044, Z3110054), the Fundamental Research Funds for the Central Universities (2011FZA6005 and 2012FZA601).

Author information

Authors and Affiliations

  1. Agronomy Department, Key Laboratory of Crop Germplasm Resource of Zhejiang Province, Zhejiang University, Hangzhou, 310058, China

    Xiaoli Jin, Shengguan Cai, Lingzhen Ye & Guoping Zhang

  2. Centre for Plants and Environment, School of Natural Sciences, University of Western Sydney, Richmond, NSW, 2753, Australia

    Zhonghua Chen

  3. Tasmanian Institute of Agricultural Research, University of Tasmania, Launceston, TAS, Australia

    Meixue Zhou

Authors
  1. Xiaoli Jin
    View author publications

    Search author on:PubMed Google Scholar

  2. Shengguan Cai
    View author publications

    Search author on:PubMed Google Scholar

  3. Lingzhen Ye
    View author publications

    Search author on:PubMed Google Scholar

  4. Zhonghua Chen
    View author publications

    Search author on:PubMed Google Scholar

  5. Meixue Zhou
    View author publications

    Search author on:PubMed Google Scholar

  6. Guoping Zhang
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to Guoping Zhang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jin, X., Cai, S., Ye, L. et al. Association of HvLDI with limit dextrinase activity and malt quality in barley. Biotechnol Lett 35, 639–645 (2013). https://doi.org/10.1007/s10529-012-1106-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-012-1106-1

Keywords

Profiles

  1. Zhonghua Chen View author profile
  2. Meixue Zhou View author profile