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Impact of lignins isolated from pretreated lignocelluloses on enzymatic cellulose saccharification

Biotechnology Letters volume 35, pages 189–195 (2013)Cite this article

Abstract

Lignins were enzymatically isolated from corn stover and wheat straw samples and subjected to hydrothermal or wet oxidation pretreatments for enzyme adsorption experimentations. Lignin contents of the isolates ranged from 26 to 71 % (w/w); cellulose ranged from 3 to 22 % (w/w); xylan from 0.7 to 6 % (w/w) and ash was from 5.8 to 30 % (w/w). ATR-IR analyses indicated significant and similar levels of calcium in all lignin isolates. Commercial cellulase adsorption studies showed that the presence of these lignins had no significant impact on the total amount of adsorbed enzyme in cellulose and cellulose–lignin systems. Consequently, the presence of the lignins had minimal effect, if any, on enzymatic cellulose conversion. Furthermore, this result, coupled with significant calcium levels in the isolated lignins, supports previous work suggesting lignin–calcium complexes reduce enzyme–lignin interactions.

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Affiliations

  1. Department of Forest & Landscape, Faculty of Science, University of Copenhagen, Rolighedsvej 23, 1958, Frederiksberg, Denmark

    Søren Barsberg, Michael Joseph Selig & Claus Felby

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  1. Søren Barsberg
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  2. Michael Joseph Selig
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  3. Claus Felby
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Correspondence to Michael Joseph Selig.

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Barsberg, S., Selig, M.J. & Felby, C. Impact of lignins isolated from pretreated lignocelluloses on enzymatic cellulose saccharification. Biotechnol Lett 35, 189–195 (2013). https://doi.org/10.1007/s10529-012-1061-x

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  • DOI: https://doi.org/10.1007/s10529-012-1061-x

Keywords

  • Adsorption
  • Biofuels
  • Calcium
  • Cellulase inhibition
  • Lignocellulose