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In vitro immunomodulatory properties of osteogenic and adipogenic differentiated mesenchymal stem cells isolated from three inbred mouse strains

Biotechnology Letters volume 35, pages 135–142 (2013)Cite this article

Abstract

Mesenchymal stem cells (MSCs) are used for cell-based therapies because of their immunomodulatory properties. The immunomodulatory properties of adipogenic (AD) and osteogenic (OS) differentiated adipose tissue-derived MSCs (AD-MSCs) isolated from BALB/c, C57BL/6, and DBA mice were compared. Splenocytes proliferation was suppressed in the presence of AD-MSCs conditioned media in all mice. After OS differentiation, BALB/c AD-MSCs produced higher levels of TGF-β and IL-17 and lower levels of NO than AD-MSCs isolated from C57BL/6 and DBA mice. In addition, OS differentiated AD-MSCs isolated from DBA mice produced lower levels of IL-10 than AD-MSCs isolated from C57BL/6 and DBA mice. After in vitro AD and OD differentiation, AD-MSCs isolated from each mouse produced higher levels of NO and IDO than undifferentiated cells. Additionally, AD-MSCs isolated from C57BL/6 and DBA mice produced higher levels of NO than AD-MSCs isolated from BALB/c mice. Adipose tissue-derived MSCs thus retain their immunomodulatory properties after in vitro OS and AD differentiation in a strain-dependent manner.

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Acknowledgments

We acknowledge funding for this study provided by Tarbiat Modares University and the Iranian Council of Stem Cell Technology.

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Affiliations

  1. Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Ale-Ahmad Avenue, P.O. Box 14115-331, Tehran, Iran

    Seyed Mahmoud Hashemi, Zuhair Mohammad Hassan, Ali Akbar Pourfathollah & Sara Soudi

  2. Stem Cell Biology Department, Stem Cell Technology Research Center, Tehran, Iran

    Sara Soudi & Abbas Shafiee

  3. Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Ale-Ahmad Avenue, P.O. Box 14115-331, Tehran, Iran

    Masoud Soleimani

Authors
  1. Seyed Mahmoud Hashemi
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  2. Zuhair Mohammad Hassan
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  3. Ali Akbar Pourfathollah
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  4. Sara Soudi
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  5. Abbas Shafiee
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  6. Masoud Soleimani
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Correspondence to Zuhair Mohammad Hassan or Masoud Soleimani.

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Hashemi, S.M., Hassan, Z.M., Pourfathollah, A.A. et al. In vitro immunomodulatory properties of osteogenic and adipogenic differentiated mesenchymal stem cells isolated from three inbred mouse strains. Biotechnol Lett 35, 135–142 (2013). https://doi.org/10.1007/s10529-012-1051-z

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  • DOI: https://doi.org/10.1007/s10529-012-1051-z

Keywords

  • Conditioned media
  • Differentiation
  • Immunomodulatory properties
  • Mesenchymal stem cells
  • Mouse strains