In vitro selection of peptide aptamers with affinity to single-wall carbon nanotubes using a ribosome display
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A ribosome display from a diverse random library was applied for selecting peptide aptamers with high binding affinity to single-wall carbon nanotubes (SWCNTs). The selected peptide aptamer bound to and solubilized SWCNTs more strongly than did the peptide aptamer selected by a phage display method reported previously, and more strongly than other commonly used organic surfactants. The fluorescence spectrum of this aptamer showed a red shift upon interaction with SWCNTs but circular dichroism spectroscopy did not show any significant difference between the presence or absence of SWCNT binding.
KeywordsAptamer In vitro selection Peptide Ribosome display Single-wall carbon nanotubes
- Chattopadhyay A, Rawat SS, Kelkar DA, Ray S, Chakrabarti A (2003) Organization and dynamics of tryptophan residues in erythroid spectrin: novel structural features of denatured spectrin revealed by the wavelength-selective fluorescence approach. Protein Sci 12(11):2389–2403PubMedCrossRefGoogle Scholar
- Hara S, Liu M, Wang W, Xu M, Li Z, Ito Y (2011) Stabilized ribosome display for in vitro selection. In: Jackson RH, Douthwaite JA (eds) Methods in Molecular Biology, vol 805. Humana Press, Cambridge, pp 59–73Google Scholar
- Tomanek D (2008) Advanced topics in the synthesis, structure, properties and applications—Foreword. Top Appl Phys. 111:5–9Google Scholar