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Protective effect of whey protein hydrolysates against hydrogen peroxide-induced oxidative stress on PC12 cells

Biotechnology Letters volume 34, pages 2001–2006 (2012)Cite this article

Abstract

The protective effect of whey protein hydrolysates (WPHs) against H2O2-induced oxidative damage on rat pheochromocytoma line 12 (PC12) cells was studied. Whey protein was hydrolyzed by pepsin and trypsin and purified by macrospore absorption resins. PC12 cells were pretreated with WPHs (from 369 to 1,980 Da) at different concentrations for 2 h, then washed and incubated with 100 μM H2O2 in the presence of WPHs for another 24 h. With 100–400 μg WPH/ml the viable cells increased by 20–30 % when incubated with H2O2 suggesting that they may play a role as antioxidant in foods.

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References

  • Beermann C, Euler M, Herzberg J, Stahl B (2009) Anti-oxidative capacity of enzymatically released peptides from soybean protein isolate. Eur Food Res Technol 229:637–644

    Article  CAS  Google Scholar 

  • Chen HM, Muramoto K, Yamauchi F, Nokihara K (1996) Antioxidant activity of designed peptides based on the antioxidative peptide isolated from digests of a soybean protein. J Agric Food Chem 44:2619–2623

    Article  Google Scholar 

  • Ito Y, Oumi S, Nagasawa T, Nishizawa N (2006) Oxidative stress induces phosphoenolpyruvate carboxykinase expression in HeIIE cells. Biosci Biotechnol Biochem 70:2191–2198

    PubMed  Article  CAS  Google Scholar 

  • Kim HJ, Lee KW, Kim MS, Lee HJ (2008) Piceatannol attenuates hydrogen-peroxide- and peroxynitrite-induced apoptosis of PC12 cells by blocking down-regulation of Bcl-XL and activation of JNK. J Nutr Biochem 19:459–466

    PubMed  Article  CAS  Google Scholar 

  • Lu RR, Qian P, Sun Z, Zhou XH, Chen TP, He JF, Zhang H, Wu JP (2010) Hempseed protein derived antioxidative peptides: purification, identification and protection from hydrogen peroxide-induced apoptosis in PC12 cells. Food Chem 123:1210–1218

    Article  CAS  Google Scholar 

  • Moure A, Domínguez H, Parajó JC (2006) Antioxidant properties of ultrafiltration-recovered soy protein fractions from industrial effluents and their hydrolysates. Process Biochem 41:447–456

    Article  CAS  Google Scholar 

  • Peña-Ramos EA, Xiong YL (2003) Whey and soy protein hydrolysates inhibit lipid oxidation in cooked pork patties. Meat Sci 64:259–263

    PubMed  Article  Google Scholar 

  • Peng XY, Xiong YL, Kong BH (2009) Antioxidant activity of peptide fractions from whey protein hydrolysates as measured by electron spin resonance. Food Chem 113:196–201

    Article  CAS  Google Scholar 

  • Pihlanto A (2006) Antioxidative peptides derived from milk proteins. Int Dairy J 16:1306–1314

    Article  CAS  Google Scholar 

  • Sakanaka S, Tachibana Y (2006) Active oxygen scavenging activity of egg–yolk protein hydrolysates and their effects on lipid oxidation in beef and tuna homogenates. Food Chem 95:243–249

    Article  CAS  Google Scholar 

  • Sargent JM, Taylor CG (1989) Appraisal of the MTT assay as a rapid test of chemosensitivity in acute myeloid leukaemia. Brit J Cancer 60:206–210

    PubMed  Article  CAS  Google Scholar 

  • Shimada K, Fujikawa K, Yahara K, Nakamura T (1992) Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J Agric Food Chem 40:945–948

    Article  CAS  Google Scholar 

  • Sinha R, Radha C, Prakash J, Kaul P (2007) Whey protein hydrolysate: functional properties, nutritional quality and utilization in beverage formulation. Food Chem 101:1484–1491

    Article  CAS  Google Scholar 

  • Sun C, Shan CY, Gao XD, Tan RX (2005) Protection of PC12 cells from hydrogen peroxide-induced injury by EPS2, an exopolysaccharide from a marine filamentous fungus Keissleriella sp. YS4108. J Biotechnol 115:137–144

    PubMed  Article  CAS  Google Scholar 

  • Wu HC, Chen HM, Shiau CY (2003) Free amino acids and peptides as related to antioxidant properties in protein hydrolysates of mackerel (Scomber austriasicus). Food Res Int 36:949–957

    Article  CAS  Google Scholar 

  • Yildirim A, Mavi A, Kara AA (2001) Determination of antioxidant and antimicrobial activities of Rumex crispus L. extracts. J Agric Food Chem 49:4083–4089

    PubMed  Article  CAS  Google Scholar 

  • Zhu KX, Zhou HM, Qian HF (2006) Antioxidant and free radical-scavenging activities of wheat germ protein hydrolysates (WGPH) prepared with alcalase. Proc Biochem 41:1296–1302

    Article  CAS  Google Scholar 

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Acknowledgments

We are grateful for the financial support by the State Natural Sciences Foundation Monumental Projects (Nos. 31071491 and 30870727), Program for New Century Excellent Talents in University (NCET-09-0436) from the People’s Republic of China, and the Natural Science Foundation of Jiangsu Province (No. BK2010156).

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, Jiangsu, China

    Qiu-Xiang Zhang, Yu-Fang Ling, Zhen Sun & Rong-Rong Lu

  2. Jiangsu Institute of Nuclear Medicine, Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, 20 Qian Rong Road, Wuxi, 214063, Jiangsu, China

    Li Zhang & Hui-Xin Yu

  3. Dairy Training Institute, P.O. Box 449, Naivasha, 20117, Kenya

    Samuel Mburu Kamau

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  1. Qiu-Xiang Zhang
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  2. Yu-Fang Ling
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  3. Zhen Sun
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  4. Li Zhang
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  5. Hui-Xin Yu
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  6. Samuel Mburu Kamau
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  7. Rong-Rong Lu
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Correspondence to Rong-Rong Lu.

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Zhang, QX., Ling, YF., Sun, Z. et al. Protective effect of whey protein hydrolysates against hydrogen peroxide-induced oxidative stress on PC12 cells. Biotechnol Lett 34, 2001–2006 (2012). https://doi.org/10.1007/s10529-012-1017-1

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  • DOI: https://doi.org/10.1007/s10529-012-1017-1

Keywords

  • Antioxidant peptide
  • Hydrogen peroxide
  • Radical scavenging activity
  • PC12 cells
  • Reactive oxygen species