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Immobilization of glucansucrase for the production of gluco-oligosaccharides from Leuconostoc mesenteroides

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Abstract

Glucansucrase from Leuconostoc mesenteroides was immobilized in 1 % (w/v) with sodium alginate to produce oligosaccharides. Glucansucrase gave three activity bands of approx. 240, 178, and 165 kDa after periodic acid-Schiff staining with sucrose. The immobilized enzyme had 40 % activity after ten batch reactions at 30 °C and 75 % activity after a month of storage at 4 °C, which is six times more stable than the free enzyme. Immobilized enzyme was more stable at lower (3.5−4.5) and higher (6.5−7.0) pH ranges and higher temperatures (35−40 °C) compared with the free enzyme. Immobilized and free glucansucrase were employed in the acceptor reaction with maltose and each produced gluco-oligosaccharide ranging from trisaccharides to homologous pentasaccharides.

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Acknowledgments

The research work was supported by a project grant from Department of Biotechnology, Ministry of Science and Technology, New Delhi, India to AG.

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Authors and Affiliations

  1. Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, 781 039, Assam, India

    Damini Kothari, Rwivoo Baruah & Arun Goyal

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  1. Damini Kothari
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  2. Rwivoo Baruah
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  3. Arun Goyal
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Correspondence to Arun Goyal.

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Kothari, D., Baruah, R. & Goyal, A. Immobilization of glucansucrase for the production of gluco-oligosaccharides from Leuconostoc mesenteroides . Biotechnol Lett 34, 2101–2106 (2012). https://doi.org/10.1007/s10529-012-1014-4

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  • DOI: https://doi.org/10.1007/s10529-012-1014-4

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