Abstract
ECO-orf27 associated with the cluster of ECO-0501 (LW01) from Amycolatopsis orientalis is deduced to encode a type II thioesterase. Disruption of ECO-orf27 reduced LW01 production by 95 %. Complementation of the disrupted mutant with intact ECO-orf27 restored the production of LW01 suggesting that ECO-orf27 is crucial for LW01 biosynthesis. ECO-TE I, the gene encoding type I thioesterase from LW01 polyketide synthases, cannot complement ECO-orf27 deficient mutant distinguishing ECO-orf27 from type I thioesterase gene. Type II thioesterase gene pikAV from Streptomyces venezuelae could complement ECO-orf27 in A. orientalis indicating that the two genes are equivalent in their function. Overexpression of ECO-orf27 resulted in a 20 % increase in LW01 production providing an alternative approach for yield improvement.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Banskota AH, McAlpine JB, Sorensen D, Ibrahim A, Aouidate M, Piraee M, Alarco AM, Farnet CM, Zazopoulos E (2006) Genomic analyses lead to novel secondary metabolites. J Antibiot 59(9):533–542
Butler AR, Bate N, Cundliffe E (1999) Impact of thioesterase activity on tylosin biosynthesis in Streptomyces fradiae. Chem Biol 6(5):287–292
Claxton HB, Akey DL, Silver MK, Admiraal SJ, Smith JL (2009) Structure and functional analysis of RifR, the type II thioesterase from the rifamycin biosynthetic pathway. J Biol Chem 284(8):5021–5029
Ding X, Tian Y, Chiao J, Zhao G, Jiang W (2003) Stability of plasmid pA387 derivatives in Amylcolatopsis mediterranei producing rifamycin. Biotechnol Lett 25(19):1647–1652
Donadio S, Monciardini P, Sosio M (2007) Polyketide synthases and nonribosomal peptide synthetases: the emerging view from bacterial genomics. Nat Prod Rep 24(5):1073–1109
Du L, Lou L (2010) PKS and NRPS release mechanisms. Nat Prod Rep 27(2):255–278
Gust B, Challis GL, Fowler K, Kieser T, Chater KF (2003) PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin. Proc Natl Acad Sci 100(4):1541–1546
Hertweck C (2009) The biosynthetic logic of polyketide diversity. Angew Chem Int Ed 48(26):4688–4716
Kieser T, Bibb MJ, Buttner MJ, Chater KF, Hopwood DA (2000) Practical Streptomyces genetics. The John Innes Foundation, Norwich
Kim BS, Cropp TA, Beck BJ, Sherman DH, Reynolds KA (2002) Biochemical evidence for an editing role of thioesterase II in the biosynthesis of the polyketide pikromycin. J Biol Chem 277(50):48028–48034
Lal R, Khanna R, Dhingra N, Khanna M, Lal S (1998) Development of an improved cloning vector and transformation system in Amycolatopsis mediterranei (Nocardia mediterranei). J Antibiot 51(2):161–169
McAlpine (2005) Polyene polyketides and methods of production. WO Patent 2005/024025A1, 17 Mar 2005
Staunton J, Weissman KJ (2001) Polyketide biosynthesis: a millennium review. Nat Prod Rep 18(4):380–416
Acknowledgments
The research is granted by the National S&T Major Special Project on Major New Drug Innovation (2009ZX9302-004) and National Natural Science Foundation (NFSC30672558).
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Shen, Y., Huang, H., Zhu, L. et al. Type II thioesterase gene (ECO-orf27) from Amycolatopsis orientalis influences production of the polyketide antibiotic, ECO-0501 (LW01). Biotechnol Lett 34, 2087–2091 (2012). https://doi.org/10.1007/s10529-012-1010-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-012-1010-8