A non-rotational, computer-controlled suspension bioreactor for expansion of umbilical cord blood mononuclear cells

Abstract

The proliferation and differentiation characteristics of umbilical cord blood mononuclear cells were examined in a non-rotational suspension bioreactor with a fishtail mixer. The system consisted of a glass vessel, a mixer that moved vertically, a data acquisition and control system to continuously monitor pH, temperature and dissolved O2. The bioreactor provided superior expansion of total HSCs and not total cell number, as well as expression of stemness-related genes which followed with increasing in number of colony-forming cells during 14 days of culture compared to T -lask culture. Vertical agitation thus reduces the total cell number, which may be related to increased shear stress, but has no effect on HSC function.

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Acknowledgments

We are very grateful to Dr. Vousogh taghi Dizaj A. and Dr. Shahverdi A.H. for valuable supporting for this project. The research was supported by the Royan Institute.

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Correspondence to Marzieh Ebrahimi.

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Shayan, N., Ebrahimi, M., Beiki, B. et al. A non-rotational, computer-controlled suspension bioreactor for expansion of umbilical cord blood mononuclear cells. Biotechnol Lett 34, 2125–2131 (2012). https://doi.org/10.1007/s10529-012-0993-5

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Keywords

  • Static culture
  • Suspension bioreactor
  • Umbilical cord blood
  • Vertical mixing