Biotechnology Letters

, Volume 34, Issue 11, pp 2125–2131 | Cite as

A non-rotational, computer-controlled suspension bioreactor for expansion of umbilical cord blood mononuclear cells

  • Niloufar Shayan
  • Marzieh EbrahimiEmail author
  • Bahareh Beiki
  • Ehsan Janzamin
Original Research Paper


The proliferation and differentiation characteristics of umbilical cord blood mononuclear cells were examined in a non-rotational suspension bioreactor with a fishtail mixer. The system consisted of a glass vessel, a mixer that moved vertically, a data acquisition and control system to continuously monitor pH, temperature and dissolved O2. The bioreactor provided superior expansion of total HSCs and not total cell number, as well as expression of stemness-related genes which followed with increasing in number of colony-forming cells during 14 days of culture compared to T -lask culture. Vertical agitation thus reduces the total cell number, which may be related to increased shear stress, but has no effect on HSC function.


Static culture Suspension bioreactor Umbilical cord blood Vertical mixing 



We are very grateful to Dr. Vousogh taghi Dizaj A. and Dr. Shahverdi A.H. for valuable supporting for this project. The research was supported by the Royan Institute.

Supplementary material

10529_2012_993_MOESM1_ESM.doc (13 kb)
Supplementary material 1 (DOC 12 kb)


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Niloufar Shayan
    • 1
  • Marzieh Ebrahimi
    • 1
    • 2
    Email author
  • Bahareh Beiki
    • 2
  • Ehsan Janzamin
    • 2
  1. 1.Department of Regenerative Medicine, Cell Research Center, Royan Institute for Stem Cell Biology and Technology, Royan Cord Blood BankACECRTehranIran
  2. 2.Department of Stem Cells and Developmental Biology, Cell Research Center, Royan Institute for Stem Cell Biology and TechnologyACECRTehranIran

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