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Analysis of histone deacetylase inhibitor-induced responses in human periodontal ligament fibroblasts

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Abstract

Periodontal ligament (PDL) fibroblasts play critical roles in the regeneration of periodontal tissues damaged by periodontitis. Histone deacetylase inhibitors (HDIs) have been suggested to be potential tools in tissue engineering. The feasibility of using the HDI, sodium butyrate (NaB) for periodontal regeneration was examined by evaluating its effect on the osteogenic differentiation of human PDL fibroblasts and its modulation of the inflammatory responses to lipopolysaccharide (LPS). NaB did not cause significant cell death at 100 μM but promoted the expression of the osteoblast phenotype (Runx2, osterix, osteocalcin, and bone sialoprotein). NaB significantly inhibited the LPS-induced production of reactive oxygen species and the expression of pro-inflammatory cytokines (IL-1β and TNF-α). These results suggest that HDIs can offer a potential therapeutic agent for periodontal regeneration.

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Acknowledgments

This study was supported by a grant of the Korean Health Technology R&D Project, Ministry for Health, Welfare and Family Affairs, Republic of Korea (A100948).

Conflict of interest

The authors report no conflicts of interest related to this study.

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Authors and Affiliations

  1. Department of Periodontology, School of Dentistry, Seoul National University, Seoul, 110-749, Republic of Korea

    Tae-Il Kim

  2. Department of Molecular Genetics, Dental Research Institute and BK21 Program, School of Dentistry, Seoul National University, Seoul, 110-749, Republic of Korea

    Ji-Eun Han, Hong-Moon Jung, Joung-Hwan Oh & Kyung Mi Woo

  3. Department of Dental Pharmacology and Therapeutics, School of Dentistry, Seoul National University, Seoul, 110-749, Republic of Korea

    Kyung Mi Woo

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  1. Tae-Il Kim
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  2. Ji-Eun Han
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  3. Hong-Moon Jung
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Correspondence to Kyung Mi Woo.

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Kim, TI., Han, JE., Jung, HM. et al. Analysis of histone deacetylase inhibitor-induced responses in human periodontal ligament fibroblasts. Biotechnol Lett 35, 129–133 (2013). https://doi.org/10.1007/s10529-012-0992-6

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  • DOI: https://doi.org/10.1007/s10529-012-0992-6

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