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Isolation and functional analysis of Thmfs1, the first major facilitator superfamily transporter from the biocontrol fungus Trichoderma harzianum

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Abstract

A novel major facilitator superfamily (MFS) transporter gene, Thmfs1, was isolated from Trichoderma harzianum (T. harzianum). A Thmfs1 over-expressing mutant displayed enhanced antifungal activity and fungicide tolerance, while the Thmfs1 disruption mutant showed the opposite trend. Trichodermin production in Thmfs1 disruption group (185 mg l−1) was decreased by less than 17 % compared to the parental strain, suggesting that Thmfs1 is not mainly responsible for trichodermin secretion. Real-time PCR showed that Thmfs1 transcript level could be induced by a certain range of trichodermin concentrations, while expression of Tri5, encoding a trichodiene synthase, was strongly inhibited under these conditions. To our knowledge, Thmfs1 is the first MFS transporter gene identified in T. harzianum.

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Acknowledgments

This research was financially supported by Zhejiang Provincial Natural Foundation of China (Grant no. Y307128), and Key Science and Technology Project of Zhejiang Province (Grant no. 2008C02007-2), which are greatly acknowledged.

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  1. Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Sciences, China Jiliang University, 258 Xueyuan Street, Xiasha, Hangzhou, 310018, Zhejiang, China

    Mu Liu, Jun Liu & Wei Min Wang

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  1. Mu Liu
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  2. Jun Liu
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  3. Wei Min Wang
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Correspondence to Jun Liu.

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Liu, M., Liu, J. & Wang, W.M. Isolation and functional analysis of Thmfs1, the first major facilitator superfamily transporter from the biocontrol fungus Trichoderma harzianum . Biotechnol Lett 34, 1857–1862 (2012). https://doi.org/10.1007/s10529-012-0972-x

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  • DOI: https://doi.org/10.1007/s10529-012-0972-x

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