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Methyl jasmonate and yeast extract stimulate mitragynine production in Mitragyna speciosa (Roxb.) Korth. shoot culture

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Abstract

Mitragynine is a pharmacologically-active terpenoid indole alkaloid found in Mitragyna speciosa leaves. Treatment with methyl jasmonate (10 μM) for 24 h and yeast extract (0.1 mg/ml) for 12 h were the optimum conditions of elicitation of mitragynine accumulation in a M. speciosa shoot culture. The former elicitor gave 0.11 mg mitragynine/g dry wt. Tryptophan decarboxylase and strictosidine synthase mRNA levels were enhanced in accordance with mitragynine accumulation.

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References

  • El-Sayed M, Verpoorte R (2007) Catharanthus terpenoid indole alkaloids: biosynthesis and regulation. Phytochem Rev 6:277–305

    Article  CAS  Google Scholar 

  • Goklany S, Loring RH, Glick J, Lee-Parsons CWT (2009) Assessing the limitations to terpenoid indole alkaloid biosynthesis in Catharanthus roseus hairy root cultures through gene expression profiling and precursor feeding. Biotechnol Prog 25(5):1289–1296

    Article  PubMed  Google Scholar 

  • Janchawee B, Keawpradub N, Chittrakarn S, Prasettho S, Wararatananurak P, Sawangjaroen K (2007) A high-performance liquid chromatographic method for determination of mitragynine in serum and its application to a pharmacokinetic study in rats. Biomed Chromatogr 21:176–183

    Article  PubMed  CAS  Google Scholar 

  • Jansen KLR, Prast CJ (1988) Ethanopharmacology of Kratom and the Mitragyna alkaloids. J Ethnopharmacol 23:115–119

    Article  PubMed  CAS  Google Scholar 

  • Jumali SS, Said IM, Baharum SN, Ismail I, Rahman ZA, Zainal Z (2011a) Molecular cloning and characterization of strictosidine synthase, a key gene in biosynthesis of mitragynine from Mitragyna speciosa. Afr J Biotechnol 10(68):15238–15244

    Article  CAS  Google Scholar 

  • Jumali SS, Said IM, Ismail I, Zainal Z (2011b) Genes induced by high concentration of salicylic acid in Mitragyna speciosa. Aust J Crop Sci 5(3):296–303

    CAS  Google Scholar 

  • Knobloch KH, Hansen B, Berlin J (1981) Medium induced formation of indole alkaloids and concomitant changes of interrelated enzyme activities in cell suspension cultures of Catharanthus roseus. Z Naturforsch 36c:40–43

    CAS  Google Scholar 

  • Memelink J, Verpoorte R, Kijne JW (2001) ORCAnization of jasmonate-responsive gene expression in alkaloid metabolism. Trends Plant Sci 6(5):212–218

    Article  PubMed  CAS  Google Scholar 

  • Nagakura N, Rüffer M, Zenk MH (1979) The biosynthesis of monoterpenoid indole alkaloids from strictosidine. JCS Perkin I:2308–2312

    Article  Google Scholar 

  • Takayama H (2004) Chemistry and pharmacology of analgesic indole alkaloids from the Rubiaceous plant, Mitragyna speciosa. Chem Pharm Bull 52:916–928

    Article  PubMed  CAS  Google Scholar 

  • Thongpradichote S, Matsumoto K, Tohda M, Takayama H, Aimi N, Sakai S, Watanabe H (1998) Identification of opioid receptor subtypes in antinociceptive actions of supraspinally-administered mitragynine in mice. Life Sci 62:1371–1378

    Article  PubMed  CAS  Google Scholar 

  • Tikhomiroff C, Jolicoeur M (2002) Screening of Catharanthus roseus secondary metabolites by high-performance liquid chromatography. J Chromatogr A 995:87–93

    Google Scholar 

  • Yamazaki Y, Urano A, Sudo H, Kitajima M, Takayama H, Yamazaki M, Aimi N, Saito K (2003) Metabolic profiling of alkaloids and strictosidine synthase activity in camptothecin producing plants. Phytochemistry 62:461–470

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

Authors thank the Prince of Songkla University (PSU) (Grant No. PHA520146S), the Graduate School of PSU, the Thailand Research Fund and The Commission of Higher Education (TRF-CHE; Grant No. RMU4980023) for financial supports. We would like to thank Dr. Brian Hodgson for assistance with the English.

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Authors and Affiliations

  1. Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Juraithip Wungsintaweekul, Jutarat Choo-malee, Tossaton Charoonratana & Niwat Keawpradub

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  1. Juraithip Wungsintaweekul
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  2. Jutarat Choo-malee
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  3. Tossaton Charoonratana
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  4. Niwat Keawpradub
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Correspondence to Juraithip Wungsintaweekul.

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Wungsintaweekul, J., Choo-malee, J., Charoonratana, T. et al. Methyl jasmonate and yeast extract stimulate mitragynine production in Mitragyna speciosa (Roxb.) Korth. shoot culture. Biotechnol Lett 34, 1945–1950 (2012). https://doi.org/10.1007/s10529-012-0968-6

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  • DOI: https://doi.org/10.1007/s10529-012-0968-6

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