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Construction of self-cloning, indigenous wine strains of Saccharomyces cerevisiae with enhanced glycerol and glutathione production

Abstract

To improve wine taste and flavor stability, a novel indigenous strain of Saccharomyces cerevisiae with enhanced glycerol and glutathione (GSH) production for winemaking was constructed. ALD6 encoding an aldehyde dehydrogenases of the indigenous yeast was replaced by a GPD1 and CUP1 gene cassette, which are responsible for NAD-dependent glycerol-3-phosphatase dehydrogenase and copper resistance, respectively. Furthermore, the α-acetohydroxyacid synthase gene ILV2 of the indigenous yeast was disrupted by integration of the GSH1 gene which encodes γ-glutamylcysteine synthetase and the CUP1 gene cassette. The fermentation capacity of the recombinant was similar to that of the wild-type strain, with an increase of 21 and 19 % in glycerol and GSH production. No heterologous DNA was harbored in the recombinant in this study.

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Acknowledgments

This study was supported by grant from China Agriculture Research System (CARS-30-jg-03). All the authors declare no interest conflict in this study.

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Correspondence to Yan-Lin Liu or Zhao-Yue Wang.

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Hao, RY., Liu, YL., Wang, ZY. et al. Construction of self-cloning, indigenous wine strains of Saccharomyces cerevisiae with enhanced glycerol and glutathione production. Biotechnol Lett 34, 1711–1717 (2012). https://doi.org/10.1007/s10529-012-0954-z

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  • DOI: https://doi.org/10.1007/s10529-012-0954-z

Keywords

  • Glutathione
  • Glycerol
  • Indigenous yeast
  • Saccharomyces cerevisiae
  • Self-cloning
  • Wine