Abstract
Optimal hydrolytic activity of β-glucosidase from Dictyoglomus turgidum for the ginsenoside Rd was at pH 5.5 and 80 °C, with a half-life of ~11 h. The enzyme hydrolysed β-linked, but not α-linked, sugar moieties of ginsenosides. It produced the rare ginsenosides, aglycon protopanaxadiol (APPD), compounds Y, and Mc, via three unique transformation pathways: Rb1 → Rd → F2 → compound K → APPD, Rb2 → compound Y, and Rc → compound Mc. The enzyme converted 0.5 mM Rb2 and 0.5 mM Rc to 0.5 mM compound Y and 0.5 mM compound Mc after 3 h, respectively, with molar conversion yields of 100 %.
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Acknowledgments
This work was supported by the Basic Research Lab program (No. 2010-0019306), the National Research Foundation, the Ministry of Education, Science and Technology, and by the Next-Generation BioGreen 21 Program grant, Rural Development Administration, Republic of Korea.
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Lee, GW., Kim, KR. & Oh, DK. Production of rare ginsenosides (compound Mc, compound Y and aglycon protopanaxadiol) by β-glucosidase from Dictyoglomus turgidum that hydrolyzes β-linked, but not α-linked, sugars in ginsenosides. Biotechnol Lett 34, 1679–1686 (2012). https://doi.org/10.1007/s10529-012-0949-9
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DOI: https://doi.org/10.1007/s10529-012-0949-9