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Expression and purification of human TAT-p53 fusion protein in Pichia pastoris and its influence on HepG2 cell apoptosis

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Abstract

P53 is an attractive target in molecular cancer therapeutics because of its critical role in regulating cell cycle arrest and apoptosis. The limitations in the development of p53-based cancer therapeutic strategy include its inefficient transmission through cell membrane of tumor cells and low protein yields in the expression system. In the present study, p53 was fused with HIV TAT protein, which can cross cell membranes, and expressed by Pichia pastoris. Stable production of Tat-p53 was achieved. After being transduced with Tat-p53 protein, the growth of cancer cell line, HepG2, was inhibited by increased apoptosis in culture. This expression system could thus be utilized to produce human Tat-p53 fusion protein.

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Acknowledgments

This work was kindly supported by Shengyuan Biotechnology Co., Ltd., Changchun, P.R. China.

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Authors and Affiliations

  1. Department of Biological Engineering, College of Pharmacy, Jilin University, 1266 Fu Jin Road, Changchun, 130021, China

    Haowei Yan, Nan Liu, Xinmin Zhang, Hao Xu, Bing Shao & Weiqun Yan

  2. Department of General Surgery, Changchun Children’s Hospital, 69 Bei An Road, Changchun, 130061, China

    Zhenghong Zhao

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  1. Haowei Yan
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  2. Nan Liu
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  3. Zhenghong Zhao
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  4. Xinmin Zhang
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  5. Hao Xu
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  6. Bing Shao
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  7. Weiqun Yan
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Correspondence to Weiqun Yan.

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Haowei Yan and Nan Liu have contributed equally to this work.

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Yan, H., Liu, N., Zhao, Z. et al. Expression and purification of human TAT-p53 fusion protein in Pichia pastoris and its influence on HepG2 cell apoptosis. Biotechnol Lett 34, 1217–1223 (2012). https://doi.org/10.1007/s10529-012-0905-8

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  • DOI: https://doi.org/10.1007/s10529-012-0905-8

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