Abstract
A method for isolating high purity and quantity RNA from Agaricus bisporus which is rich in proteins, carbohydrate, fiber and secondary metabolites, is described. RNA was extracted from mycelium, primordia, sporophores at two development stages and two post-harvest storage stages as well as from pileipellis, inner cap, gill and stipe of the mature sporophore. The A260/A230 and A260/A280 ratios of isolated RNA from fruiting bodies were both ~2 and the yield was about 200 μg/g fresh wt (FW). The yield of RNA from mycelium was approx. 100 μg/g FW. High quality RNA was also extracted from fruiting body tissues of Lentinus edodes, Pleurotus ostreatus, Flammulina velutipes and Pleurotus eryngii with yields from 130 to 225 μg/g FW. RNA extracted from all samples was intact, as demonstrated by gel electrophoresis and was suitable for downstream molecular applications, including RT-PCR and qPCR.
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Acknowledgments
We thank Prof. Avtar Krishan Handa, Purdue University to help us to revise grammar. This work was supported by the National Natural Science Foundation of China (No. 31071623 and 30671471) and the Commonweal Industry Scientific Researcher Project from the Ministry of Agriculture (No. 200803033).
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Meng, Dm., Shen, L., Zhang, Xh. et al. Isolation of high quality and yield of RNA from Agaricus bisporus with a simple, inexpensive and reliable method. Biotechnol Lett 34, 1315–1320 (2012). https://doi.org/10.1007/s10529-012-0903-x
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DOI: https://doi.org/10.1007/s10529-012-0903-x