Abstract
To develop recombinant epitope vaccines against foot-and-mouth disease virus (FMDV), genes coding for six recombinant proteins (rP1–rP6) consisting of different combinations of B cell and T cell epitope from VP1 capsid protein (VP1) of type O FMDV were constructed and the 3D structure of these proteins analyzed. This revealed a surface-exposed RGD sequence of B cell epitopes in all six recombinant proteins as that in VP1 of FMDV and rP1, rP2 and rP4 globally mimicked the backbone conformation of the VP1. rP1, rP2 and rP4 stimulated guinea pigs to produce higher level of neutralizing antibodies capable of protecting suckling mice against FMDV challenge. rP1 stimulated cattle to produce FMDV-neutralizing antibody. The data suggest that an efficient recombinant epitope vaccine against FMDV should share local similarities with the natural VP1 of FMDV.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abdul-Hamid NF, Hussein NM, Wadsworth J et al (2011) Phylogeography of foot-and-mouth disease virus types O and A in Malaysia and surrounding countries. Infect Genet Evol 11:320–328
Bittle JL, Houghten RA, Alexander H et al (1982) Protection against foot-and-mouth disease by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequence. Nature 298:30–33
Collen T, Dimarchi R, Doel TR (1991) A T cell epitope in VP1 of foot-and-mouth disease virus is immunodominant for vaccinated cattle. J Immunol 146:749–755
DiMarchi R, Brooke G, Gale C et al (1986) Protection of cattle against foot-and-mouth disease by a synthetic peptide. Science 232:639–641
Du Y, Li Y, He H et al (2008) Enhanced immunogenicity of multiple-epitopes of foot-and-mouth disease virus fused with porcine interferon α in mice and protective efficacy in guinea pigs and swine. J Virol Methods 149:144–152
Gao SD, Du JZ, Chang HY et al (2010) B Cell epitopes within VP1 of type O foot-and-mouth disease virus for detection of viral antibodies. Virol Sin 25:18–26
He C, Wang H, Wei H et al (2010) A recombinant truncated FMDV 3AB protein used to better distinguish infected and vaccinated cattle. Vaccine 28:3435–3439
King A, Underwood B, McCahon D et al (1981) Biochemical identification of the viruses causing the 1981 outbreak of foot-and-mouth disease virus in UK. Nature 293:479–480
Li G, Chen W, Yan W et al (2004) Comparison of immune responses against foot-and-mouth disease virus induced by fusion proteins using the swine IgG heavy chain constant region or beta-galactosidase as a carrier of immunogenic epitopes. Virology 328:274–281
Luis LR, Marvin JG (2009) Foot and mouth disease virus vaccines. Vaccine 27:90–94
Luizinho C, Mario SB, Mauro PM et al (2005) Granulocyte-macrophage colony-stimulating factor does not increase the potency or efficacy of a foot-and-mouth disease virus subunit vaccine. Pesqui Vet Bras 25:150–158
Mateu MG, Camarero JA, Giralt E et al (1995) Direct evaluation of the immunodominance of a major antigenic site of foot-and-mouth disease virus in a natural host. Virology 206:298–306
Reed LJ, Muench H (1938) A simple method of estimating fifty percent endpoints. Am J Hyg 27:493–497
Rodriguez LL, Barrera J, Kramer E et al (2003) A synthetic peptide containing the consensus sequence of the G–H loop region of foot-and mouth disease virus type-O VP1 and a promiscuous T-helper epitope induces peptide-specific antibodies but fails to protect cattle against viral challenge. Vaccine 21:3751–3756
Shao JJ, Wong CK, Lin T et al (2011) Promising multiple-epitope recombinant vaccine against foot-and-mouth disease virus type O in swine. Clin Vaccine Immunol 18:143–149
Sirskyi D, Diaz-Mitoma F, Golshani A et al (2011) Innovative bioinformatic approaches for developing peptide-based vaccines against hypervariable viruses. Immunol Cell Biol 89:81–89
Sobrino F, Blanco E, García-Briones M et al (1999) Synthetic peptide vaccines: foot-and-mouth disease virus as a model. Dev Biol Stand 101:39–43
Wei JC, Huang YZ, Zhong DK et al (2010) Design and evaluation of a multi-epitope peptide against Japanese encephalitis 3 virus infection in BALB/c mice. Biochem Biophys Res Commun 396:787–792
Zamorano PI, Wigdorovitz A, Pérez Filgueira DM et al (1998) Induction of anti foot and mouth disease virus T and B cell responses in cattle immunized with a peptide representing ten amino acids of VP1. Vaccine 16:558–563
Zhang Q, Yang YQ, Zhang ZY et al (2002) Immunogenicity of a recombinant fusion protein of tandem repeat epitopes of foot-and-mouth disease virus type Asia 1 for guinea pigs. Acta Virol 46:1–9
Acknowledgments
We gratefully thank the Baoling Bio-pharmaceutical limited Corporation, Jinyu Group (Huhehot, Inner Mongolia, China) for conducting the experiments of cattle immunization and FMDV challenge. This work was supported by the Program (2009GJB10015) of Technologists in Service of Business from National Department of Technology, China.
Author information
Authors and Affiliations
Corresponding authors
Additional information
Mingli Fang and Jianli Li contributed equally to this work.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Fang, M., Li, J., Wang, H. et al. Correlation between efficacy and structure of recombinant epitope vaccines against bovine type O foot and mouth disease virus. Biotechnol Lett 34, 839–847 (2012). https://doi.org/10.1007/s10529-012-0856-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-012-0856-0