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Preparation of encapsulated alliinase in alginate microparticles

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Abstract

Alliinase (alkylsulphenate lyase, EC 4.4.1.4), which catalyses the production of allicin, was immobilized in alginate microparticles. Addition of pyridoxal 5′-phosphate to the microparticles enhanced alliinase activity. Encapsulated alliinase were significantly higher (30 and 22%, respectively) than those of non-encapsulated alliinase at 60°C and at pH 2. Therefore, microencapsulation of alliinase with alginate can offer an effective way of sustaining enzyme activity during oral administration and passage through the stomach.

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Acknowledgments

This study was supported by a Grant of Korea University and the Korea Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea (A050376).

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Authors and Affiliations

  1. School of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, Korea

    J. A. Ko, Y. L. Lee, H. J. Jeong & H. J. Park

  2. LG Household & Health Care, Daejeon, 305-343, Korea

    Y. L. Lee

  3. Department of Packaging Science, Clemson University, Clemson, SC, 29634-0370, USA

    H. J. Park

Authors
  1. J. A. Ko
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  2. Y. L. Lee
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  3. H. J. Jeong
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  4. H. J. Park
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Corresponding author

Correspondence to H. J. Park.

Additional information

J. A. Ko and Y. L. Lee equally contributed to this research as co-first authors.

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Ko, J.A., Lee, Y.L., Jeong, H.J. et al. Preparation of encapsulated alliinase in alginate microparticles. Biotechnol Lett 34, 515–518 (2012). https://doi.org/10.1007/s10529-011-0791-5

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  • DOI: https://doi.org/10.1007/s10529-011-0791-5

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