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Construction of multi-functional extracellular matrix proteins that inhibits migration and tube formation of endothelial cells

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Abstract

Artificial extracellular matrix (ECM) proteins have been designed that have strong cell-adhesive activity and active functional units that inhibit network formation among vascular endothelial cells. A laminin-derived sequence (YIGSR) that blocks migration of vascular endothelial cells was designed to incorporate into an elastin-derived structural unit. The designed ECM fusion protein also had a cell-adhesive RGDN sequence that conferred an intense migration-inhibitory effect. The resultant ECM showed cell-adhesive activity superior to that of the synthetic YIGSR peptide and it blocked the migration of vascular endothelial cells. Furthermore, the designed ECM inhibited the angiogenic activity of a collagen gel. The engineering strategy of designing multi-functional ECM proteins could be applied to support novel tissue engineering techniques.

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Acknowledgment

We thank the members of Nakamura Bio-printing Project at Kanagawa Academy of the Science and Technology (KAST, Japan) for technical support with endothelial angiogenesis.

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Authors and Affiliations

  1. Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, 226-8501, Japan

    Makiko Nakamura, Masayasu Mie & Eiry Kobatake

  2. Faculty of Lifescience and Engineering, University of Toyama, 3190 Gohuku, Toyama-shi, Toyama, 930-8555, Japan

    Makoto Nakamura

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  1. Makiko Nakamura
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  2. Masayasu Mie
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  3. Makoto Nakamura
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  4. Eiry Kobatake
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Correspondence to Eiry Kobatake.

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Nakamura, M., Mie, M., Nakamura, M. et al. Construction of multi-functional extracellular matrix proteins that inhibits migration and tube formation of endothelial cells. Biotechnol Lett 34, 1571–1577 (2012). https://doi.org/10.1007/s10529-011-0788-0

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  • DOI: https://doi.org/10.1007/s10529-011-0788-0

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