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Expression and purification of two alternative peptides for mechano-growth factor in Escherichia coli

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Two genes, MGFc 40 and MGFc 24, encoding different E peptides of mechano-growth factor (MGF), were obtained by a four-step PCR strategy and subcloned into pRSETC and pGEX-6p-1. Recombinant MGFc40 protein (4 mg l−1) was expressed and purified by affinity chromatography using His60 Ni Superflow. Recombinant MGFc24 protein was purified using a glutathione-Sepharose 4B column. After enzymatic cleavage of the GST-tail, 1 mg MGFc24 protein l−1 was obtained. MGFc40 and MGFc24, which are involved in proliferation and differentiation, stimulated cell proliferation and inhibited cell differentiation of C2C12 cells.

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This work was supported by grants from the National Natural Science Foundation of China (No. 31040018). This research project was supported by the Shanxi Scholarship Council of China (2010-2012), Natural Sciences Foundation of Shanxi (2009021035-2) and International technical cooperation projects of Shanxi (2011081058).

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Correspondence to Li Song.

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Song, L., Zhu, WS., Dong, JL. et al. Expression and purification of two alternative peptides for mechano-growth factor in Escherichia coli . Biotechnol Lett 34, 231–237 (2012).

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