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Stromal cell derived factor-1α enhances bone formation based on in situ recruitment: a histologic and histometric study in rabbit calvaria

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Abstract

Histological methods were used to assess whether in situ recruitment using stromal cell derived factor-1α (SDF-1α) enhances bone formation. Four defects were created in the calvarias of 16 rabbits and filled with: (1) a blood clot only (group C); (2) autogenous bone particles (AB, 0.2 ml) (group AB); (3) AB (0.1 ml) + bone marrow derived stromal stem cells (group ABC); or (4) AB (0.1 ml) + SDF-1α (group ABS). Bone formation was significantly greater in groups AB and ABC compared with group ABS after 2 weeks (P < 0.05). Bone formation was similar between groups AB, ABC, and ABS after 4 weeks (P > 0.05). SDF-1α is a promising candidate for in situ recruitment in bone regeneration.

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Acknowledgments

This study was supported in part by a grant (100334) from the National Natural Science Foundation of China. The authors would like to thank Chunyang Xi and Yufu Wang for providing technical assistance.

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Authors and Affiliations

  1. Department of Orthopedic Surgery, The First Affiliated Hospital, Harbin Medical University, 23Youzheng St, Nan Gang District, Harbin, 150001, People’s Republic of China

    Xiaoqi Liu, Shifeng Zhou, Yanjing Li & Jinglong Yan

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  1. Xiaoqi Liu
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  2. Shifeng Zhou
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  3. Yanjing Li
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  4. Jinglong Yan
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Correspondence to Jinglong Yan.

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Liu, X., Zhou, S., Li, Y. et al. Stromal cell derived factor-1α enhances bone formation based on in situ recruitment: a histologic and histometric study in rabbit calvaria. Biotechnol Lett 34, 387–395 (2012). https://doi.org/10.1007/s10529-011-0757-7

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  • DOI: https://doi.org/10.1007/s10529-011-0757-7

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