Skip to main content
SpringerLink
Log in

Use of Pichia fermentans and Candida sp. strains for the biological treatment of stored olive mill wastewater

  • Original Research Paper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Of 105 isolates screened for growth on plates containing olive mill wastewater (OMW), five were selected and identified as Pichia fermentans (Y1, Y4) and Candida sp. (Y2, Y11, and Y18). On the basis of their ability to use phenol at 716 mg l−1, strains Y2 (15% reduction) and Y4 (18% reduction) were then used to detoxify stored OMW under various operational conditions. Yeast treatment of OMW increased the pH and, in the best conditions (aeration and no glucose addition), the COD decreased (47%) and phytotoxicity was also decreased (56%) probably due to the changes in the composition of phenolic compounds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Amaral C, Lucas MS, Coutinho J, Crespí AL, Anjos MR, Pais C (2008) Microbiological and physicochemical characterization of olive mill wastewaters from a continuous olive mill in northeastern Portugal. Bioresour Technol 99:215–223

    Article  Google Scholar 

  • Assas N, Ayed L, Marouani L, Hamdi M (2002) Decolorization of fresh and stored-black olive mill wastewaters by Geotrichum candidum. Proc Biochem 38:361–365

    Article  CAS  Google Scholar 

  • Ben Sassi A, Boularbah A, Jaouad A, Walker G, Boussaid A (2006) A comparison of olive mill wastewater (OMW) from three different processes in Marocco. Proc Biochem 41:74–78

    Article  CAS  Google Scholar 

  • Ben Sassi A, Ouazzani N, Walker GM, Ibnsouda S, El Mzibri M, Boussaid A (2008) Detoxification of olive mill wastewater by Maroccan yeast isolates. Biodegradation 19:337–346

    Article  PubMed  CAS  Google Scholar 

  • Casa R, D’Annibale A, Pieruccetti F, Stazi SR, Giovannozzi Sermanni G, Lo Cascio B (2003) Reduction of the phenolic components in olive-mill wastewater by an enzymatic treatment and its impact on durum wheat (Triticum durum Desf.) germinability. Chemosphere 50:959–966

    Article  PubMed  CAS  Google Scholar 

  • Cereti CF, Rossini F, Federici F, Quarantino D, Vassilev N, Fenice M (2004) Reuse of microbially treated olive mill wastewater as fertiliser for wheat (Triticum durum Desf.). Bioresour Technol 91:135–140

    Article  Google Scholar 

  • Esteve-Zarzoso B, Belloch C, Uruburu F, Querol A (1999) Identification of yeasts by RFLP analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers. Int J Syst Bacteriol 49:329–337

    Article  PubMed  CAS  Google Scholar 

  • Fakharedine N, El Hajjouji H, Ait Baddi G, Revel JC, Hafidi M (2006) Chemical and spectroscopic analysis of organic matter transformation during aerobic digestion of olive-mill waste-waters. Proc Biochem 41:398–404

    Article  CAS  Google Scholar 

  • Gonçalves C, Lopes M, Ferreira P, Belo I (2009) Biological treatment of olive mill wastewater by non-conventional yeasts. Bioresour Technol 100:3759–3763

    Article  PubMed  Google Scholar 

  • Jaouani A, Sayadi S, Vanthournhout M, Penninckx MJ (2003) Potent fungi for decolourisation of olive mill wastewaters. Enzyme Microb Technol 33:802–809

    Article  CAS  Google Scholar 

  • Kurtzman CP, Robnett CJ (1998) Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie Leeuwenhoek 73:331–371

    Article  PubMed  CAS  Google Scholar 

  • Lanciotti R, Gianotti A, Baldi D, Angrisani R, Suzzi G, Mastrocola D, Guerzoni ME (2005) Use of Yarrowia lipolytica strains for the treatment of olive mill wastewater. Bioresour Technol 96:317–322

    Article  PubMed  CAS  Google Scholar 

  • Papanikolaou S, Galiotou-Panayotou M, Fakas S, Komaitis M, Aggelis G (2008) Citric acid production by Yarrowia lipolytica cultivated on olive-mill-wastewater based media. Bioresour Technol 99:2419–2428

    Article  PubMed  CAS  Google Scholar 

  • Paredes C, Cegarra J, Roig A, Sánchez-Monedero MA, Bernal MP (1999) Characterization of olive-mill wastewater (alpechín) and its sludge for agricultural purposes. Bioresour Technol 67:111–115

    Article  CAS  Google Scholar 

  • Sinigaglia M, Di Benedetto N, Bevilaqua A, Corbo MR, Capece A, Romano P (2010) Yeasts isolated from olive mill wastewaters from southern Italy: technological characterization and potential use for phenol removal. Appl Microbiol Biotechnol 87:2345–2354

    Article  PubMed  CAS  Google Scholar 

  • Yan J, Jianping W, Hongmei L, Suliang Y, Zongding H (2005) The biodegradation of phenol at high initial concentration by the yeast Candida tropicalis. Biochem Eng J 24:243–247

    Article  Google Scholar 

  • Zucconi F, Pera A, Forte M, De Bertoldi M (1981) Evaluating toxicity of immature compost. Biocycle 22:54–57

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dipartimento SAIFET, sez. di Microbiologia Alimentare, Industriale e Ambientale, Università Politecnica delle Marche, Via Brecce Bianche, 60131, Ancona, Italy

    Manuela Taccari & Maurizio Ciani

Authors
  1. Manuela Taccari
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Maurizio Ciani
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Maurizio Ciani.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 45 kb)

Supplementary material 2 (DOCX 112 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Taccari, M., Ciani, M. Use of Pichia fermentans and Candida sp. strains for the biological treatment of stored olive mill wastewater. Biotechnol Lett 33, 2385–2390 (2011). https://doi.org/10.1007/s10529-011-0708-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-011-0708-3

Keywords

Search

Navigation