Abstract
A protocol for the in vitro propagation of Drosera aliciae to increase the yield of the naphthoquinone, ramentaceone, was developed. The highest micropropagation coefficient was obtained using half-strength Murashige–Skoog medium supplemented with 0.4 μM 6-benzyladenine (BA). The genetic fidelity and stability of the regenerated plants was confirmed with RAPD markers. The activity of the isolated ramentaceone was determined against four human tumor cell lines: U937, HeLa, MCF-7, HCT-116 with the highest cytotoxic activity towards the leukemic U937 cell line with an IC50 value of 3.2 μM.
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Acknowledgments
This work was supported by the grant of the Ministry of Science and Higher Education no. 3021/B/P01/2009/36. This publication is also financed by European Social Fund as a part of the project “Educators for the elite—integrated training program for PhD students, post-docs and professors as academic teachers at University of Gdansk” within the framework of Human Capital Operational Programme, Action 4.1.1, Improving the quality of educational offer of tertiary education institutions. We express our gratitude to Prof. M. Stobiecki (Institute of Bioorganic Chemistry, PAS, Poznan) for the isolation and identification of ramentaceone.
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Kawiak, A., Królicka, A. & Łojkowska, E. In vitro cultures of Drosera aliciae as a source of a cytotoxic naphthoquinone: ramentaceone. Biotechnol Lett 33, 2309–2316 (2011). https://doi.org/10.1007/s10529-011-0700-y
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DOI: https://doi.org/10.1007/s10529-011-0700-y