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Applicability of thermo-alkali-stable and cellulase-free xylanase from a novel thermo-halo-alkaliphilic Bacillus halodurans in producing xylooligosaccharides

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Abstract

An alkaliphilic, moderately thermophilic and halophilic bacterial isolate capable of producing a high titer of extracellular thermo-alkali-stable, cellulase-free endoxylanase was isolated from the paper mill effluents. It was identified as Bacillus halodurans. The purified xylanase was active from pH 7 to 12 and 30 to 100°C with optimal activity at pH 9.0 and 80°C. It had T1/2 values of 40 and 15 min at 70 and 80°C, respectively. Activity was stimulated by dithiothreitol but strongly inhibited by N-bromosuccinimide. Its action on birchwood xylan and agro-residues liberated xylooligosaccharides of 2–7 degree of polymerization, and thus, the mode of action is similar to endoxylanases of the family 10 glucoside hydrolases.

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Acknowledgments

We are grateful to the Ministry of Environment & Forests and Department of Biotechnology, Government of India for providing financial assistance while carrying out the work presented in the manuscript.

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  1. Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India

    Vikash Kumar & T. Satyanarayana

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  1. Vikash Kumar
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  2. T. Satyanarayana
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Correspondence to T. Satyanarayana.

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Kumar, V., Satyanarayana, T. Applicability of thermo-alkali-stable and cellulase-free xylanase from a novel thermo-halo-alkaliphilic Bacillus halodurans in producing xylooligosaccharides. Biotechnol Lett 33, 2279–2285 (2011). https://doi.org/10.1007/s10529-011-0698-1

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  • DOI: https://doi.org/10.1007/s10529-011-0698-1

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