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Biotechnology Letters

, Volume 33, Issue 6, pp 1249–1255 | Cite as

Increased resistance to cucumber mosaic virus (CMV) in Lilium transformed with a defective CMV replicase gene

  • Pejman Azadi
  • Ntui Valentaine Otang
  • Hasthanasombut Supaporn
  • Raham Sher Khan
  • Dong Poh Chin
  • Ikuo Nakamura
  • Masahiro MiiEmail author
Original Research Paper

Abstract

Lilium cv Acapulco was transformed with a defective cucumber mosaic virus (CMV) replicase gene (CMV2-GDD) construct using Agrobacterium tumefaciens. Four lines were analyzed for gene expression and resistance to CMV-O strain. Expression of the CMV2-GDD gene in the transgenic plants was confirmed by reverse transcription PCR (RT-PCR). When these four lines were mechanically inoculated with CMV-O, no signal of coat protein (CP) messages using RT-PCR was detected in newly produced leaves of two transgenic lines. Dot-immunobinding assay (DIBA) of CP was performed to examine the presence of the CMV in the newly produced leaves of challenged plants. Results, similar to those obtained with RT-PCR of the CP messages, were observed in DIBA. Therefore, our results imply that the two lines show increased levels of resistance to CMV, and CMV-GDD replicase gene is an effective construct that has protection against CMV in Lilium.

Keywords

Agrobacterium-mediated transformation Cucumber mosaic virus Lilium Replicase gene 

Notes

Acknowledgments

This work was supported by Ministry of Education, Culture, Sport, Science and Technology of the Japanese Government (MONBUKAGAKUSHO) P. Azadi is the recipient of a Ph.D. fellowship from Ministry of Education, Science and Culture of Japan.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Pejman Azadi
    • 1
  • Ntui Valentaine Otang
    • 1
  • Hasthanasombut Supaporn
    • 1
  • Raham Sher Khan
    • 1
  • Dong Poh Chin
    • 1
  • Ikuo Nakamura
    • 1
  • Masahiro Mii
    • 1
    Email author
  1. 1.Laboratory of Plant Cell Technology, Graduate School of HorticultureChiba UniversityMatsudo CityJapan

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