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A modified electro-transformation method for Bacillus subtilis and its application in the production of antimicrobial lipopeptides

Biotechnology Letters volume 33, pages 1047–1051 (2011)Cite this article

Abstract

A modified electroporation method using trehalose is presented for the transformation of Bacillus subtilis. The new method improved the transformation efficiency of B. subtilis nearly 2,000-fold compared with the usual method, giving 4 × 105 transformants/μg DNA. Using this method, B. subtilis was engineered to improve production of antimicrobial lipopeptides and produced 1.8-fold more surfactin and 2.9-fold more fengycin.

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Acknowledgments

We thank the National Natural Science Foundation of China (No.30871753) for their financial support of this research.

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Authors and Affiliations

  1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China

    Guoqiang Cao, Xiaohui Zhang, Lei Zhong & Zhaoxin Lu

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  1. Guoqiang Cao
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  2. Xiaohui Zhang
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  3. Lei Zhong
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  4. Zhaoxin Lu
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Correspondence to Zhaoxin Lu.

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Cao, G., Zhang, X., Zhong, L. et al. A modified electro-transformation method for Bacillus subtilis and its application in the production of antimicrobial lipopeptides. Biotechnol Lett 33, 1047–1051 (2011). https://doi.org/10.1007/s10529-011-0531-x

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  • DOI: https://doi.org/10.1007/s10529-011-0531-x

Keywords

  • Antimicrobial lipopeptides
  • Bacillus subtilis
  • Electroporation
  • Trehalose