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Regioselective deglycosylation of onion quercetin glucosides by Saccharomyces cerevisiae

Abstract

Bioconversion of quercetin glucosides using four generally recognized as safe (GRAS) organisms (Aspergillus oryzae, Bacillus subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae) was evaluated by measuring changes in the levels of quercetin compounds of onion. Of the four organisms, S. cerevisiae increased the content of quercetin-3-O-β-d-glucoside (III; isoquercitrin) and quercetin (IV), whereas decreasing quercetin-3,4′-O-β-d-glucoside (I) and quercetin-4′-O-β-d-glucoside (II). Also, S. cerevisiae converted authentic compound I to III, and II to IV, respectively. These results suggest that S. cerevisiae can be used to increase the levels of isoquercitrin (III), the most bioavailable quercetin compound in onion.

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References

  • Appleton J (2010) Evaluating the bioavailability of isoquercetin. Nat Med J 2:1–6

    Google Scholar 

  • Bonaccorsi P, Carrado C, Gargiulli C, Leuzzi U (2008) Flavonol glucosides in Allium species: a comparative study by means of HPLC-DAD-ESA-MS-MS. Food Chem 107:1668–1673

    CAS  Google Scholar 

  • Day AJ, Dupont MS, Ridley S, Rhodes M, Rhodes MJC, Morgan MRA, Williamson G (1998) Deglycosylation of flavonoid and isoflavonoid glycosides by human small intestine and liver β-glucosidase activity. FEBS Lett 436:71–75

    PubMed  Article  CAS  Google Scholar 

  • Donaghy J, Kelly PF, McKay AM (1998) Detection of ferulic acid esterase production by Bacillus sp. and lactobacilli. Appl Microbiol Biotechnol 50:257–260

    PubMed  Article  CAS  Google Scholar 

  • Griffiths G, Trueman L, Crowther T, Thomas B, Smith B (2002) Onions—a global benefit to health. Phytother Res 16:603–615

    PubMed  Article  CAS  Google Scholar 

  • Kim YJ, Narayanan S, Chan KO (2010) Inhibition of influenza virus replication by plant-derived isoquercetin. Antiviral Res (in press)

  • Lombard K, Peffley E, Geoffriau E, Thompson L, Herring A (2005) Quercetin in onion (Allium cepa L.) after heat-treatment simulating home preparation. J Food Compost Anal 18:571–581

    Article  CAS  Google Scholar 

  • Price KR, Rhodes MJ (1997) Analysis of the major flavonol glycosides present in four varieties of onion (Allium cepa) and changes in composition resulting from autolysis. J Sci Food Agric 74:331–339

    Article  CAS  Google Scholar 

  • Slimestad R, Fossen T, Vågen IM (2007) Onions: a source of unique dietary flavonoids. J Agric Food Chem 55:10067–10080

    PubMed  Article  CAS  Google Scholar 

  • Takahama U, Hirota S (2000) Deglucosidation of quercetin glucosides to the aglycone and formation of antifungal agents by peroxidase-dependent oxidation of quercetin on browning of onion scales. Plant Cell Physiol 41:1021–1029

    PubMed  Article  CAS  Google Scholar 

  • Yoo KS, Lee EJ, Patil BS (2010) Quantification of quercetin glycosides in 6 onion cultivars and comparisons of hydrolysis-HPLC and spectrophotometric methods in measuring total quercetin concentrations. J Food Sci 72:160–165

    Article  Google Scholar 

Download references

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Correspondence to Hyo-Kon Chun.

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Chung, DM., Chung, YC., Maeng, P.J. et al. Regioselective deglycosylation of onion quercetin glucosides by Saccharomyces cerevisiae . Biotechnol Lett 33, 783–786 (2011). https://doi.org/10.1007/s10529-010-0501-8

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  • DOI: https://doi.org/10.1007/s10529-010-0501-8

Keywords

  • Bioconversion
  • Isoquercitrin
  • Onion
  • Quercetin
  • Saccharomyces cerevisiae