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Functionalized quantum dots to quantify NADPH and their use for NADP+-dependent biocatalyzed transformations

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Abstract

Quantum dots are semiconducting nanoparticles that can be prepared with interesting optical properties. The fluorescent properties of quantum dots are one of the key advantages for their use as optical labels for biorecognition events and biocatalytic processes. We have prepared semiconductor quantum dots conjugated with Nile Blue (NB), and demonstrate that NB-functionalized quantum dots can act as versatile probes to analyze different biocatalyzed transformations, and can be used for the quantitative detection of NADPH as well as NADH. This approach provides a new path for the optical detection of NAD(P)H and for the quantitative analysis of NAD(P)+-dependent biotransformations.

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Acknowledgments

We are grateful to Professor Jin-Woo Bae for his help. This work was supported by Basic Science Program through the National Research Foundation of Korea (KRF) funded by the Ministry of Education, Science and Technology (MEST) (no. 2010-0015218) and also by the National Research Laboratory Fund through the Nano Optical Property Laboratory.

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Authors and Affiliations

  1. Department of Chemistry and Research Institute for Basic Sciences, Kyung Hee University, Seoul, 130-701, Korea

    Min Young Kim, Yea Seul Kim, Jisu Kim & Sang Soo Hah

  2. Department of Physics, Kyung Hee University, Seoul, 130-701, Korea

    Tae Jung Kim & Young Dong Kim

Authors
  1. Min Young Kim
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  2. Yea Seul Kim
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  3. Jisu Kim
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  4. Sang Soo Hah
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  5. Tae Jung Kim
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  6. Young Dong Kim
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Corresponding author

Correspondence to Sang Soo Hah.

Additional information

M. Y. Kim and Y. S. Kim contributed equally to this work.

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Kim, M.Y., Kim, Y.S., Kim, J. et al. Functionalized quantum dots to quantify NADPH and their use for NADP+-dependent biocatalyzed transformations. Biotechnol Lett 33, 623–628 (2011). https://doi.org/10.1007/s10529-010-0476-5

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  • DOI: https://doi.org/10.1007/s10529-010-0476-5

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