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Enzymatic synthesis of sucrose octaacetate using a novel alkaline protease

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Abstract

Acylation of 0.5 g sucrose with 1.2 ml acetic anhydride was carried out in 2 ml two-solvent medium of anhydrous pyridine/n-hexane (1:1, v/v) using 0.2 g crude protease from Serratia sp. Sucrose octaacetate was the sole product and more than 90% sucrose was converted in 24 h at 30°C. The purity of sucrose octaacetate reached 100%, via a simple purification method of alcohol/water washing and centrifugation.

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Acknowledgments

This work was financially supported by the National High Technology and Development Program of China (863 Program, Grant No. 2010AA101501).

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Authors and Affiliations

  1. Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Lihu Road 1800, Wuxi, 214122, Jiangsu, China

    Guiying Li, Yujie Cai, Xiangru Liao & Jing Yin

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  1. Guiying Li
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  2. Yujie Cai
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  3. Xiangru Liao
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  4. Jing Yin
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Corresponding author

Correspondence to Xiangru Liao.

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Li, G., Cai, Y., Liao, X. et al. Enzymatic synthesis of sucrose octaacetate using a novel alkaline protease. Biotechnol Lett 33, 607–610 (2011). https://doi.org/10.1007/s10529-010-0468-5

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  • DOI: https://doi.org/10.1007/s10529-010-0468-5

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