Abstract
The shuttle vector pGYC4α (6,157 bp) was constructed based on the sigma-replicon plasmid pYC2 from Lactobacillus sakei BM5 isolated from kimchi. The vector contained inserts of the ColE1 replicon, α-amylase gene from Bacillus licheniformis containing its own signal peptide, and lactococcal promoter P32. Transformation and expression of a selection marker gene (α-amylase) with pGYC4α were demonstrated in Escherichia coli and several lactic acid bacteria (LAB). The highest α-amylase activity in LAB transformants was obtained in M17/0.25% glucose media with 0.5% CaCO3. The segregational stability of the shuttle vector in LAB was 100% for more than 100 generations in the absence of antibiotic pressure. The developed vector might be useful as a genetic tool for food industries.
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Yang, E.J., Chang, H.C. Construction and evaluation of shuttle vector, pGYC4α, based on pYC2 from Lactobacillus sakei . Biotechnol Lett 33, 599–605 (2011). https://doi.org/10.1007/s10529-010-0467-6
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DOI: https://doi.org/10.1007/s10529-010-0467-6