Abstract
The stability (half-life, t½) of the large catalase (CAT) isolated from Aspergillus terreus was decreased under acidic conditions (maximum t½ ~8.5 months at pH ≤ 6) versus alkaline conditions (t½ ~15 months at pH 8–12). Acidic conditions induce the dissociation of haem from CAT, as revealed from a reduction in the Soret peak intensity at 405 nm and an increase in the peak current at Fe3+/Fe2+ redox potentials. This increase in current is attributed to the facile electron transfer from the free haem generated on the electrode surface as a result of its disintegration from the insulating protein matrix. The haem isolated from CAT at acidic condition was reconstituted with apo-CAT at alkaline denaturing conditions to regenerate the CAT activity.
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We acknowledge the financial support from the Department of Biotechnology, Government of India, to carry out this work.
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Vatsyayan, P., Goswami, P. Acidic pH conditions induce dissociation of the haem from the protein and destabilise the catalase isolated from Aspergillus terreus . Biotechnol Lett 33, 347–351 (2011). https://doi.org/10.1007/s10529-010-0442-2
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DOI: https://doi.org/10.1007/s10529-010-0442-2