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Simultaneous extraction of carotenoids and transfructosylating enzyme from Xanthophyllomyces dendrorhous by a bead beater

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An Erratum to this article was published on 10 January 2012

Abstract

Purpose of work

Conditions for the cell disruption of Xanthophyllomyces dendrorhous by using a bead beater have been optimized with respect to maximizing the recovery of carotenoids and transfructosylating enzymes in a one-step process.

The optimal conditions for a one-step extraction of carotenoids and transfructosylating enzymes from Xanthophyllomyces dendrorhous were: 30 g dry cells/l being disintegrated with 0.5 mm diam. glass beads at 10°C for 15 min using a bead beater. More than 90% yeast disruption and, when extracted with hexane/ethyl acetate, 28.6 mg carotenoids/l in the organic solvent phase and 440 U transfructosylating enzyme activity/mg protein in the water phase were obtained.

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Acknowledgment

The authors are grateful for the financial support of National Science Council, Taiwan, People’s Republic of China (NSC95-2313-E-005-054).

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, National Chung Hsing University, 250 Kuokuang Road, Taichung city, Taiwan, 40227, People’s Republic of China

    Shu-Chih Lee & Tony J. Fang

Authors
  1. Shu-Chih Lee
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  2. Tony J. Fang
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Corresponding author

Correspondence to Tony J. Fang.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s10529-011-0803-5

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Lee, SC., Fang, T.J. Simultaneous extraction of carotenoids and transfructosylating enzyme from Xanthophyllomyces dendrorhous by a bead beater. Biotechnol Lett 33, 109–112 (2011). https://doi.org/10.1007/s10529-010-0400-z

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  • DOI: https://doi.org/10.1007/s10529-010-0400-z

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